Author/Editor		Gartner, Urška; Jermol, Urška
Title		Izražanje encimov ciklooksigenaza-1 in ciklooksigenaza-2 v zdravem srcu in v srčnem infarktu pri človeku
Type		monografija
Place		Ljubljana
Publisher		Univerza v Ljubljani, Medicinska fakulteta
Publication year		2005
Volume		str. 40
Language		slo
Abstract		Background. Cyclooxygenase (COX) is a key enzyme in prostanoid synthesis which play an imporiant role in many cell functions. COX exists in two isofonns: COXI and COX-2. COX-1 is constitutively expressed in many tissues in which it produces prostanoids for normal physiological functions. COX-2 is an inducibile isoform, its synthesis can be induced by proinflammatory and mitogenic stimuli, leading to prostanoid overproduction in inflammatory, ischemic and neoplastic tissue. AIM and Hypothesis. Our aim was to analyse the expression of both COX isoforms in the normal human heari and in myocardial infarction (MI). We hypothesised that COX-1 is expressed mostly or exclusively in the normal heart and COX-2 in MI. Methods. Our study included autopsy samples of heart tissue divided into 2 groups: one consisted of heart tissue samples from 10 healthy persons who died in car accidents, and the other consisted of heart tissue samples from 35 patients with MI. Tissue samples were stained with HE and PTAH for demonstration of MI. Por detection of COX-1 and COX-2, immunohistochemistry was used. Results. In the normal heart, COX-1 was found in blood vessels, endocardium and nerve fibres; in MI, it was detected in inflammatory cells, capillaries and myofibroblasts in granulation and fibrous tissue. No COX-1 positivity was found in cardiomyocytes. COX-2 was not observed in the normal heart. Positive reaction was observed in cardiomyocytes in all cases of MI, the staining being most intensive in early MI (up to 2 days old). In MI between 2 and 7 days old, positive reaction was additionally found in neutrophil granulocytes in the interstitium. In MI between 1 and 4 weeks old, rare inflammatory cells, capillaries and myofibroblasts in granulation and fibrous tissue were also labeled. (Abstract truncated at 2000 characters)
Descriptors		MYOCARDIAL INFARCTION PROSTAGLANDIN-ENDOPEROXIDE SYNTHASE AUTOPSY