Author/Editor		Virant-Klun, Irma; Tomaževič, Tomaž; Vrtačnik-Bokal, Eda; Vogler, Andrej; Krsnik, Mladen; Meden-Vrtovec, Helena
Title		Increased ammonium in culture medium reduces the development of human embryos to the blastocyst stage
Type		članek
Source		Fertil Steril
Vol. and No.		Letnik 85, št. 2
Publication year		2006
Volume		str. 526-8
Language		eng
Abstract		The objective of this prospective study was to evaluate the effect of ammonium accumulated in sequential media and determined by enzymatic spectrophotometric method on the blastocyst development in 281 human embryos from 100 stimulated and natural in vitro fertilization (IVF) cycles. Ammonium concentration was increased in 62% of cycles and was correlated negatively with the blastocyst development after classical IVF, but not after intracytoplasmic sperm injection (ICSI).
Descriptors		FERTILIZATION IN VITRO AMMONIUM COMPOUNDS BLASTOCYST SPERMATOZOA CULTURE MEDIA PROSPECTIVE STUDIES SPECTROPHOTOMETRY OSMOLAR CONCENTRATION EMBRYO