Author/Editor		Šimenc, Janez; Ružić-Sabljić, Eva; Avguštin, Gorazd
Title		Differentiation of Borrelia burgdorferi sensu lato species by temperature gradient gel electrophoresis
Type		članek
Source		Electrophoresis
Vol. and No.		Letnik 27
Publication year		2006
Volume		str. 1322-30
Language		eng
Abstract		Borrelia burgdorferi sensu lato is a multispecies complex of pathogenic spirochetes, causing Lyme borreliosis. Due to clinical, epidemiological, and taxonomical implications, there is a need for identification of isolated Borrelia strains. In the present study, we have optimized TGGE for B. burgdorferi sensu lato species differentiation and the results were compared with two reference methods, namely PFGE and restriction of 5S-23S intergenic space region PCR product. A differentiation of 8. garinii, 8. afzelii, and 8. burgdorferi senso stricto species with TGGE was possible and intraspecies variation was detected. Results compared between TGGE, PFGE, and restriction of 5S-23S intergenic space region PCR product showed no difference in specificity of species identification.
Descriptors		BORRELIA BURGDORFERI DNA, BACTERIAL TEMPERATURE ELECTROPHORESIS, POLYACRYLAMIDE GEL ELECTROPHORESIS, GEL, PULSED-FIELD