Author/Editor		Jurič, Damijana Mojca; Miklič, Špela; Čarman-Kržan, Marija
Title		Monoaminergic neuronal activity up-regulates BDNF synthesis in cultured neonatal rat astrocytes
Type		članek
Source		Brain Res
Vol. and No.		Letnik 1108, št. 1
Publication year		2006
Volume		str. 54-62
Language		eng
Abstract		Astrocytes as an active part of the tripartite synapse can respond to the synaptically released neurotransmitters. Because brain-derived neurotrophic factor (BDNF) is produced by astrocytes, in addition to neurons, we focused our present study on the regulatory effects of monoamines noradrenaline (NA), serotonin (5-HT), and dopamine (DA) on the synthesis of BDNF protein in rat neonatal astrocytes from specific brain regions (cortex, cerebellum). All tested neurotransmitters are able to potently and transiently increase BDNF cellular contents; their maximal effects are dose and time dependent and differ between the two brain regions. In cultured cortical astrocytes, NA (1miM; 6h) elevates BDNF levels by a 4-fold, 5-HT (1miM; 4h) by a 2.3-fold, and DA (150miM; 4h) by a 2.2-fold. In cerebellar astrocytes, NA (1miM; 4h) increases BDNF content by a 4.7-fold, 5-HT (1miM; 4h) by a 1.7-fold, and DA (150miM; 4h) by a 1.4-fold. The initial increase in the BDNF levels return to basal levels when incubation with monoamines is extended beyond 12 h (for 5-HT) or 24 h (for NA and DA). Our results confirm the involvement of monoaminergic systems in the regulation of BDNF production in astrocytes and suggest the existence of a positive reciprocal interaction between monoaminergic neuronal activity and astrocytic neurotrophic support in neuronastrocyte crosstalk, which has a dynamic role in mediating neuronal plasticity and trophic functions in brain.
Descriptors		ASTROCYTES BRAIN-DERIVED NEUROTROPHIC FACTOR NOREPINEPHRINE SEROTONIN DOPAMINE CELLS, CULTURED CEREBRAL CORTEX CEREBELLUM DOSE-RESPONSE RELATIONSHIP, DRUG KINETICS RATS