Author/Editor     Bizjak, Bojana
Title     Semi-quantitative method for detection of fetomaternal hemorrhage gel agglutination technique
Type     članek
Source     In: Rožman P, Urlep-Šalinović V, editors. Zbornik predavanj 10 let uporabe gelske aglutinacijske metode pri tranfuzisjkih preiskavah v Sloveniji;. Maribor: Splošna bolnišnica Maribor,
Publication year     2006
Volume     str. 48-52
Language     eng
Abstract     Background: In order to prevent maternal sensibilization with the child's RhD positive red blood cells (RBC), RhD negative mothers are treated with RhD-immune globulin (RhD Ig). The standard dosage in Slovenia is 300 mig of RhD Ig in the 28th week of gestation and 150 mig of RhD Ig after delivery. 150 mig of RhD Ig suffices for a 7,5 ml of fetal RhD positive RBCs, representing an approximately 0,25% of fetal RBC subpopulation in a maternal circulation. Adequate administration of RhD Ig requires an accurate measurement of the volume of RhD positive RBC in the circulation of a mother. Therefore, we looked for a simple and rapid screening test for detection of more than 0,25% of fetal RBC subpopulation. Methods: A mother's postpartal sample is incubated with a monoclonal anti-D serum from ID-FMH Screening Test (DiaMed, Switzerland). Commercial adult RhD-negative RBC with various amounts of fetal RhD positive RBC (0.1%, 0.2% and 0.4%) of the same manufacturer are tested in parallel, to aid interpretation. After incubation, the supernatants are tested against R2R2 RBCs in order to determine the reduction of anti-D reactivity. We compared ID-FMH Screening Test results with the Kleihauer-Betke test, which is based on fetal hemoglobin's resistance to acid elution, unlike adult hemoglobin. A commercial Sure-tech Diagnostic kit was used. Results: 386 postpartum samples from RhD-negative women who delivered RhD-positive infants were tested using both tests. In four samples fetomaternal hemorrhage (FMH) exceeded 7.5 ml of fetal RBCs (15 ml of whole blood) and mothers needed more than 150 mig of RhD Ig. (Abstract trunacted at 2000 characters)
Descriptors     FETOMATERNAL TRANSFUSION
RH ISOIMMUNIZATION
AGGLUTINATION TESTS
RHO(D) IMMUNE GLOBULIN