Author/Editor		Bartolić, Miha; Humar, Primož
Title		Učinki ekvinatoksina II na morfologijo in homeostazo znotrajceličnega okolja endotelijskih celic
Type		monografija
Place		Ljubljana
Publisher		Univerza v Ljubljani, Medicinska fakulteta
Publication year		2005
Volume		str. 34
Language		slo
Abstract		Background: Equinatoxin II (EqT II) is a cytolytic basic peptide isolated from the sea anemone Actinia equina (L.). After oligomerization this actinoporin forms cation selective pores in phospholipid membranes. Lethal effects of this toxin are due to the cardiorespiratory arrest. Despite intensive investigation the direct cardiotoxic effects and mechanism of a drastic decrease of the coronary perfusion remain unclear. The role of endothelium in the mechanism of the toxins lethal effects has been discussed, but is insufficiently explained. Therefore it seems reasonable to study the possible role of endothelial cells in the decreased perfusion. An increase of the volume of those cells may increase the resistance in the microvasculature resulting in a decreased perfusion rate. Aims: The aim of the study is to assess the effects of EqT II on 1) the dynamic changes of intracellular Ca2+ activity 2) volume and morphology changes in the endothelial cell culture, and 3) the role of the composition of the extracellular fluid on those changes. Hypothesis: The main hypothesis is that the application of EqT II causes a significant increase of the endothelial cell volume. It has also been assumed that the increase of the cell volume is linked to an increased intracellular Ca2+activity which is due to the Ca2+ entry and not due to its release from the intracellular stores. (Abstract truncated at 2000 characters)
Summary		Izhodišče: Ekvinatoksin II (EqT II) je citolitični bazični peptid, izoliran iz morske vetrnice Actinia eguina (L.). Po načinu delovanja je aktinoporin, ki v plazemski membrani z oligomerizacijo dela kationsko selektivne pore. Letalni učinki EqT II so posledica kardiorespiratornega zastoja. Nepojasnjeni ostajajo neposredni kardiotoksični učinki toksina in mehanizem zmanjšanja koronarnega pretoka. Nejasna ostaja tudi vloga endotelijskih celic v mehanizmu posledične ishemije organov, predvidevamo pa, da je zoženje lumna žil v mikrocirkulaciji tudi posledica nabrekanja endotelijskih celic. Namen dela: Z našo raziskavo smo želeli preučiti 1) dinamiko sprememb znotrajcelične aktivnosti kalcija po dodatku EqT II, 2) volumske in morfološke spremembe v kulturi endotelijskih celic po dodatku EqT II, 3) vpliv sestave zunajcelične raztopine na volumske in morfološke spremembe na kulturi endotelijskih celic po dodatku EqT II. Hipoteze: Poskuse smo zasnovali tako, da smo z njimi lahko preverjali hipotezo, da po dodatku EqT 11 naraste volumen endotelijskih celic. Predvidevali smo, da je porast volumna celic povezan s porastom znotrajcelične aktivnosti kalcijevih ionov, ki je posledica vdora kalcijevih ionov v celico in ne sproščanja znotrajceličnih zalog kalcija. (Izvleček skrajšan na 2000 znakov)
Descriptors		ENDOTHELIUM, VASCULAR CNIDARIAN VENOMS CALCIUM CELL SIZE FURA-2 SEA ANEMONES CELL LINE MICROSCOPY, CONFOCAL