Author/Editor		Brvar, Miran; Finderle, Žarko; Šuput, Dušan; Bunc, Matjaž
Title		S100B protein in conscious carbon monoxide-poisoned rats treated with normobaric or hyperbaric oxygen
Type		članek
Source		Crit Care Med
Vol. and No.		Letnik 34, št. 8
Publication year		2006
Volume		str. 2228-30
Language		eng
Abstract		Objective: To evaluate S100B, an astroglial structural protein, during normobaric and hyperbaric oxygen therapy of conscious carbon monoxide (CO)-poisoned rats. So far, the usefulness of hyperbaric oxygen therapy in conscious CO-poisoned patients has been shown with neuropsychological testing. The S100B protein has been demonstrated as a possible biochemical marker and prognostic parameter in CO-poisoned rats. Design: Randomized, controlled interventional trial. Setting: University laboratory. Subjects: Male Wistar rats weighing 254 +- 14 g. Interventions: The rats were exposed to a mixture of 3,000 ppm CO in air for 60 mins. After CO exposure, the first group of eight conscious rats was exposed to ambient air for 30 mins, the second group of six conscious rats was exposed to 100% normobaric oxygen for 30 mins, and the third group of six conscious rats was exposed to 100% hyperbaric oxygen at 3 bars for 30 mins. Blood samples were taken from the jugular vein just before CO exposure and immediately after oxygen therapy. The level of consciousness was evaluated at the end of exposure, and the survival rate was monitored for 14 days. The S100B concentrations were measured with a commercial immunoluminometric assay. Measurements and Main Results: Analyses of differences in S100B levels between different kinds of therapy before and after treatment showed a global significant difference (p = .002). The post hoc test results showed that S100B levels after therapy of the first group treated with ambient air (0.16 ± 0.07 microg/L) and the second group treated with normobaric oxygen (0.19 ± 0.05 microg/L) were similar (p = .741), and both of them were significantly different, with much higher values of S100B levels after therapy, from the third group treated with hyperbaric oxygen (0.06 ± 0.03 microg/L; p = .018 and p = .002, respectively). All the rats survived. (Abstract truncated at 2000 characters)
Descriptors		CARBON MONOXIDE POISONING OXYGEN INHALATION THERAPY HYPERBARIC OXYGENATION NERVE TISSUE PROTEIN S 100 CARBOXYHEMOGLOBIN RATS, WISTAR