Author/Editor		Pinter, Bojana; Tomaževič, Tomaž; Meden-Vrtovec, Helena; Drobnič, Sašo; Bačer-Kermavner, Liljana; Virant-Klun, Irma; Kolbezen-Simoniti, Mojca; Hribernik, Alenka; Korošec, Sara; Geršak, Ksenija; Gradišek, Blanka
Title		Postopki oploditve z biomedicinsko pomočjo z darovanimi spolnimi celicami na Ginekološki kliniki Kliničnega centra v Ljubljani
Translated title		Assisted reproduction technology procedures using donated gametes at the Department of obstetrics and gynecology, University medical centre Ljubljana
Type		članek
Source		Zdrav Vestn
Vol. and No.		Letnik 75, št. 8
Publication year		2006
Volume		str. 463-8
Language		slo
Abstract		Background After the law on assisted reproductive technologies (ART) in Slovenia was legalized, donor procedures at the Department of Ob/Gyn, University Medical Center Ljubljana, were started at the end of 2001; by September 2005 22Z recipient couples have applied for the procedures: 153 couples for donor semen (DS) procedures and 68 couples for donor oocyte (DO) procedures. Methods Gametes can be donated by healthy men aged I8-55 years and healthy women aged 18 to 35 years. After donation, donor semen is frozen, and donor oocytes are fertilized with the semen of the recipient, after which embryos are frozen. If after a 6-month safety period repeated tests on sexually transmitted infections are negative, thawed semen or embryos can be used, for the recipients. Results Gametes have been donated by 97 men and 30 women. ART using DS was performed in 98 couples. Pregnancy rate per embryo transfer (ET) of fresh embryos has been 30 %; pregnancy rate per ET of thawed embryos has been 20 %. ART using DO have been performed in 46 couples. After a 6 months safety period, embryos of 38 couples have been transferred to recipients. Pregnancy rate on ET of thawed embryos has been 19 %. Conclusions Our experiences have shown, that gamete donation program can be implemented also in Slovenia and that donor procedures are equally successful as homologous procedures. In the, future we intend to consolidate the program.
Summary		Izhodišča S sprejetjem ustrezne zakonodaje v Sloveniji smo na Ginekološki kliniki Kliničnega centra v Ljubljani začeli z darovalskimi postopki konec leta 2001. Za tovrstne postopke je do septembra 2005 pri nas zaprosilo 221 neplodnih parov: 153 parov za postopke z darovanim semenom (DS) in 68 parov za postopke z darovanimi jajčnimi celicami (DO). Metode Spolne celice lahko darujejo zdravi moški, stari 18-55 let, in zdrave ženske, stare 18-35 let. Darovano seme zamrznemo, darovana jajčeca pa oplodimo s semenom partnerja prejemnice in zarodke zamrznemo. Po šestih mesecih varovalne dobe ob ponovno negativnih testih na spolno prenosljive okužbe uporabimo odmrznjeno seme oz prenesemo odmrznjene zarodke v maternico prejemnice. Rezultati Spolne celice je darovalo 97 darovalcev in 30 darovalk. Postopke zunajtelesne oploditve z DS smo opravili pri 98 parih. Delež zanositev na prenos (ET) svežih zarodkov je bil 30%, delež zanositev na ET odmrznjenih zarodkov pa 20%. Postopke zunajtelesne oploditve z DO smo opravili pri 46 parih. Po polletni varovalni dobi smo pri 38 parih že odmrznili zarodke in jih prenesli v maternico. Delež zanositev na ET je bil 19%. Zaključki Naše izkušnje so pokazale, da je darovanje spolnih celic mogoče vzpostaviti tudi v Sloveniji in da so darovalski postopki enako uspešni kot homologni postopki. V prihodnosti želimo program še utrditi.
Descriptors		FERTILIZATION IN VITRO SPERMATOZOA OOCYTES TISSUE DONORS INFERTILITY