Author/Editor     Trček, J; Toyama, H; Czuba, J; Misiewicz, A; Katsushita, K
Title     Towards understanding the acetic acid resistance in Gluconacetobacter europaeus
Type     članek
Source     In: Mendez-Vilas A, editor. Modern multidisciplinary applied microbiology: exploiting microbes and their interactions. Weinheim: Wiley-VCH,
Publication year     2006
Volume     str. 674-8
Language     eng
Abstract     High acetic acid resistance strains (up to 10 %), identified as Gluconacetobacter europaeus, Gluconacetobacter intermedius and Acetobacter ftarteurianus have been analysed in this study by different molecular-biological approaches. The profiles of membrane proteins from Ga. intermedius and Ga. europaeus harvested at different points of ethanol oxidation phase were compared. Concomitant with the increased amount of acetic acid, the expression of a ca. 95 kDa protein substantially increased. The N-terminal sequence of the protein was determined. Low temperature difference spectra of membranes from all three species showed a presence of cytochrome a1 which is a putative ba3 ubiquinol oxidase. The morphology of cells was compared by scanning electron microscopy. Further, a transposon mutagenesis procedure was established with the aim to study the acetic acid resistance on the genetic level.
Descriptors     ACETOBACTERACEAE
ACETIC ACID
MEMBRANE PROTEINS
MICROSCOPY, ELECTRON, SCANNING
ELECTRON TRANSPORT
ALCOHOL, ETHYL
MUTAGENESIS
CYTOCHROME A