| Author/Editor | Hočevar, Samo B; Živin, Marko; Milutinovič, Aleksandra; Hawlina, Marko; Hutton, Emily; Ogorevc, Božidar | |
| Title | Simultaneous in vivo measurement of dopamine, serotonin and ascorbate in the striatum of experimental rats using voltammetric microprobe | |
| Type | članek | |
| Source | Frontiers in bioscience | |
| Vol. and No. | Letnik 11 | |
| Publication year | 2006 | |
| Volume | str. 2782-9 | |
| Language | eng | |
| Abstract | The in vivo performance of a voltammetric microprobe based on overoxidized poly(1,2-phenylenediamine) coated carbon fiber microelectrode (OPPD/CFME), developed in our laboratory, is presented. For this purpose, an OPPD microprobe was stereotaxicaly implanted in the striatum of a deeply anesthetized Wistar rat for the simultaneous measurement of dopamine, serotonin and ascorbate. Furthermore, the post mortem levels of these physiologically important compounds were monitored after the rats were terminated with an overdosc of anesthetic introduced through an indwelling jugular catheter. Using cyclic (CV) and square-wave (SWV) voltammetry, the OfPD/CFME was demonstrated to exhibit efficient separation of the voltammetric signals of dopamine, serotonin and ascorbate in the presence of biological matrix, with the SWV mode alluwing more convenient detection regarding both sensitivity and selectivity. Explicit proof of in vivo dopamine detection at the OPPD/CFME was achieved via the absence of the dopamine signal in rats with unilateral lesions of nigrostriatal dopaminergic neurons, which was induced by the use of the selective dopaminergic neurotoxin 6-hydroxydopamine (6-OHDA). In stark contrast to non-treated rats, where a strong signal corresponding to 1.2 micro M dopamine was measured at ca. 90 s after the rats' death, the signal for dopamine in dopamine-depleted striatum of 6-ONDA rats was absent. A critical comparison of the in vivo performance of the OPPD/CFME to that of bare and Nation-coated carbon fiber microelectrodes, often used in neurophysiological studies, clearly showed a signifiicant advantage of the former microprobe. (Abstract truncated at 2000 characters) | |
| Descriptors | CORPUS STRIATUM ASCORBIC ACID DOPAMINE SEROTONIN RATS, WISTAR ELECTROCHEMISTRY MICROELECTRODES STEREOTAXIC TECHNIQUES |