Author/Editor		Contreras, Juan Antonio
Title		Hormone-sensitive lipase is not required for cholesteryl ester hydrolysis in macrophages
Type		članek
Source		Biochem Biophys Res Commun
Vol. and No.		Letnik 292, št. 4
Publication year		2002
Volume		str. 900-3
Language		eng
Abstract		Storage of cholesteryl esters in the cytoplasm of macrophages is one of the earliest and most ubiquitous event observed in the development of arteriosclerosis. Macrophages have an enormous capacity to uptake and store cholesterol in the form of cytosolic cholesteryl ester droplets. These stores are mobilized by the action of a neutral cholesteryl ester hydrolase (NCEH), producing free cholesterol that is either secreted to extracellular acceptors or reesterified. It has been proposed that hormone-sensitive lipase (HSL) is responsible for the NCEH activity in macrophages. The present work shows, however, that peritoneal macrophages from HSL null mice hydrolyze cytosolic stores of cholesteryl esters at a comparable rate to that of peritoneal macrophages from wild-type mice, therefore demonstrating that HSL is not the main NCEH in macrophages.
Descriptors		ADIPOSE TISSUE ARTERIOSCLEROSIS BLOTTING, WESTERN BUCLADESINE CELLS, CULTURED CHOLESTEROL ESTERASE CHOLESTEROL ESTERS CYTOPLASM HYDROLYSIS MACROPHAGES, PERITONEAL MICE MICE, INBRED STRAINS MICE, KNOCKOUT