Author/Editor		Contreras, JA; Danielsson, B; Johansson, C; Osterlund, T; Langin, D; Holm, C
Title		Human hormone-sensitive lipase: expression and large-scale purification from a baculovirus/insect cell system
Type		članek
Source		Protein Expr Purif
Vol. and No.		Letnik 12, št. 1
Publication year		1998
Volume		str. 93-9
Language		eng
Abstract		Hormone-sensitive lipase (HSL) is a key enzyme in lipid metabolism and overall energy homeostasis in mammals. It catalyzes the rate-limiting step in the hydrolysis of triglyceride stores in the adipocytes, delivering free fatty acids for their use as energy substrates. HSL activity is under acute hormonal and neural control, mediated through reversible phosphorylation of the enzyme. Emerging data from clinical studies indicate that HSL deficiency or malfunction is associated with several pathological situations in humans. In order to perform a biochemical characterization of human HSL, and to elucidate its molecular properties, purification of homogeneous protein in large amounts is required. Here, we describe the expression and purification of a catalytically active recombinant human HSL. The process allows the purification of milligram amounts of homogeneous protein, and should provide a valuable tool for a thorough molecular characterization of the enzyme.
Descriptors		AMINO ACID SEQUENCE BACULOVIRIDAE BASE SEQUENCE CELL LINE CHOLESTEROL ESTERASE CLONING, MOLECULAR DNA, COMPLEMENTARY GENE EXPRESSION MOLECULAR SEQUENCE DATA RATS RECOMBINANT PROTEINS SPODOPTERA