Author/Editor		Iglesias, A; Contreras, JA; Martinez-Pardo, M; Entrala, A; Herrera, E; Lasuncion, MA
Title		Cholesteryl ester transfer activity in lipoprotein lipase deficiency and other primary hypertriglyceridemias
Type		članek
Source		Clin Chim Acta
Vol. and No.		Letnik 221, št. 1-2
Publication year		1993
Volume		str. 73-89
Language		eng
Abstract		Cholesteryl ester transfer protein (CETP) activity was measured in d > 1.21 g/ml plasma from hypertriglyceridemic patients and compared with normolipidemic subjects. The assay consisted in measuring the specific transfer of [3H]cholesteryl oleate from a prelabelled, apo E-poor HDL fraction to VLDL after incubation at 37 degrees C in the presence of the d > 1.21 g/ml plasma sample: the lipoproteins were then separated by precipitation with dextran sulfate/Mg2+ solution. Increasing the volume of d > 1.21 g/ml plasma or purified human CETP in the assay produced linear responses in measured activity, whereas, either during incubation at 4 degrees C or in the presence of rat plasma instead of human plasma, the transfer of [3H]cholesteryl oleate to VLDL was not stimulated. Thus, the assay reflects changes in CETP in the sample and appears to be suitable for measuring CETP activity in d > 1.21 g/ml plasma. CETP activity was very similar in the two groups of normolipidemic subjects considered: adolescents (203 +/- 11 nmol esterified cholesterol transferred per 8 h/ml plasma) and adults (215 +/- 5). Patients were grouped into lipoprotein-lipase (LPL)-deficient and non-LPL-deficient according to their enzyme activity in postheparin plasma. CETP activity was highly increased in LPL-deficient, severe hyperchylomicronemic patients (430 +/- 42) and was directly correlated with VLDL levels in the non-LPL-deficient individuals. Marked differences were observed in the lipid composition of HDL and apolipoprotein A-I levels among patients and controls. In the control group, CETP activity was correlated only with HDL-triglyceride and HDL-triglyceride/apo A-I mass ratio, which is compatible with the physiological role of CETP in transferring triglyceride to HDL from other lipoprotein particles. (Abs. trunc. at 2000 ch.)
Descriptors		ADULT AGED CARRIER PROTEINS CHILD CHILD, PRESCHOOL CHOLESTEROL ESTERS HYPERTRIGLYCERIDEMIA LIPOPROTEIN LIPASE DEFICIENCY, FAMILIAL LIPOPROTEINS, HDL CHOLESTEROL LIPOPROTEINS, VLDL CHOLESTEROL TRIGLYCERIDES TRITIUM