| Author/Editor | Bocos, C; Castro, M; Orozco, E; Contreras, JA; Herrera, E | |
| Title | A rapid and sensitive method for HPLC cholesterol determination in bile | |
| Type | članek | |
| Source | Rev Esp Fisiol | |
| Vol. and No. | Letnik 48, št. 3 | |
| Publication year | 1992 | |
| Volume | str. 211-4 | |
| Language | eng | |
| Abstract | A relatively little time consuming simple method based on the treatment of bile with cholesterol oxidase and subsequent high performance liquid chromatography measurement of the 3-ketocholesterol produced in order to determine the level of the cholesterol concentration is described. The method avoids bilirubin interferences, has high reproducibility and recovery assays give 100% values. It is highly sensitive and suitable for use in the determination of cholesterol concentrations in bile and other bilirubin containing biological fluids. | |
| Descriptors | CHROMATOGRAPHY, HIGH PRESSURE LIQUID BILE CHOLESTEROL KETOCHOLESTEROLS RATS, SPRAGUE-DAWLEY REPRODUCIBILITY OF RESULTS SENSITIVITY AND SPECIFICITY TIME FACTORS |