Author/Editor		Zavašnik-Bergant, Tina; Bergant, Martina; Jeras, Matjaž; Griffiths, Gareth
Title		Different localisation of cystatin C in immature and mature dendritic cells
Translated title		Različna lokalizacija cistatina C v nezrelih in zrelih dendritičnih celicah
Type		članek
Source		Radiol Oncol
Vol. and No.		Letnik 40, št. 3
Publication year		2006
Volume		str. 183-8
Language		eng
Abstract		Background. Limited antigen degradation by proteolytic enzymes and their control by protease inhibitors represent a crucial step in generating antigenic peptides inside the endocytic pathway of antigen-presenting cells such as dendritic cells. Methods. Human dendritic cells were used as a cell model in which quantitative immunogold electron microscopy was applied in order to study endogenous protease inhibitor cystatin C. Ultrathin cryosections were prepared from immature and mature dendritic cells and labelled with specific antibody. Under the transmission electron microscope gold particles, bound to specific probe (antibody), pointed the exact localization of labelled inhibitor Results. Quantification of immunogold labelling and further statistical analysis by chi-squared test emphasized the differences in cystatin C content in different cell compartments. Conclusions. Statistically significant differences in intracellular distribution of cystatin C have been determined between immature and mature dendritic cell population.
Summary		Izhodišča. Razgradnja antigenov s proteolitskimi encimi (proteazami) v endocitozni poti antigena predstavitvenih celic (dendritičnih celic) ter njihova regulacija z inhibitorji proteaz predstavlja pomemben korak pri nastanku antigenskih peptidov. Metode. Cloveške dendritične celice so bile uporabljene kot celični model za študij proteaznega inhibitorja cistatina C. Pripravljene so bile tanke zamrznjene rezine nezrelih in zrelih dendritičnih celic ter označene s specifičnimi protitelesi za kvantitativno elektronsko mikroskopijo. Pod transmisijskim elektronskim mikroskopom so zrna koloidnega zlata, vezana na specifične sonde (protitelesa), pokazala natančno lokalizacijo označenega inhibitorj a. Rezultati. Ovrednotenje označenih celic s statističnim testom Hi-kvadrat je potrdilo razlike v vsebnosti cistatina C v različnih celičnih organelih. Zaključki. Potrjena je bila statistično značilna razlika v znotrajcelični porazdelitvi cistatina C med populacijama nezrelih in zrelih dendritičnih celic.
Descriptors		DENDRITIC CELLS MONOCYTES TUMOR NECROSIS FACTOR CRYOULTRAMICROTOMY MICROSCOPY, ELECTRON IMMUNOHISTOCHEMISTRY CYSTATINS