Author/Editor		Repše-Fokter, Alenka
Title		Primerjava morfometričnih značilnosti celic v brisih materničnega vratu in mineralne kostne gostote
Type		monografija
Place		Ljubljana
Publisher		Univerza v Ljubljani, Medicinska fakulteta
Publication year		2006
Volume		str. 77
Language		slo
Abstract		Background: Osteoporosis is a common human bone disease often accompanied with consequent fractures, which have recently been creating an increasingly significant economic burden. Early identification and treatment of the disorder are therefore very important. Whilst dual X-ray absorptiometry is a generally accepted "gold standard" for the measurement of bone mineral density, it is on the other hand to expensive for regular screening. Risk factors for osteoporosis are well known and included in a variety of questionnaires designed to help assess the existing risk level in bone mineral density measurements. The present study attempts to evaluate possible relationship between morphometric and morphologic cell characteristics in Pap smears, serum estradiol concentrations, anthropometric characteristics, and bone mineral density. Matherial and methods: The research comprised 136 women,subjected to dual x-ray absorptiometry measurement on the ocasion of routine cervical cancer screening including collection of Pap smears and measurement of body weight, height and body mass index. Of the above number, 107 subject consented to blood examination to determine serum concentrations of estradiol. Using the astereological analysis for each smear, we calculated the mean areas of 200 systematically sampled squamous cells, their nuclei, cytoplasm, and their nuclear-cytoplasmic ratio. The values were compared with T-scores, measured by dual x-ray absorptiometry. The smears were grouped into atrophic and mature cell patterns and were also compared with T-scores at hip and lumbar spine. Results: The mean cell and cytoplasmic areas were significantly lower in women with lower T-scores at femoral neck and lumbar spine (p<0,001). There was no significant relationship between the nuclear area and T-score neither at femoral neck at lumbar spine (p>0,05). (Abstract truncated at 2000 charactes)
Summary		Izhodišča: Osteoporoza postaja v svetu in pri nas vse bolj pogosta, zdravljenje njenih posledic pa predstavlja vse večje finančno breme. Zato sta zalo pomembna zgodnja prepoznava bolezni in pravočasno zdravljenje. Dvoenergijska rentgenska absorpciometrija je splošno sprejeta referenčna metoda za merjenje mineralne kostne gostote, vendar za presejenje zaradi visoke cene ni primerna. Dejavniki tveganja za osteoporozo so dobro znani in v različni meri zajeti v raznih testih za oceno tveganja. Z raziskavo smo želeli ugotoviti, ali obstaja povezava med morfometričnimi in morfološkimi značilnostmi celic v brisih materničnega vratu, koncentracijo estradiola v serumu, antropometričnimi značilnostmi in mineralno kostno gostoto. Material in metode: V raziskavi je bilo zajetih 136 žena, ki jim je bil na rednem preventivnem ginekološkem pregledu odvzet bris materničnega vratu, opravljeno merjenje mineralne kostne gostote z dvoenergijsko rentgensko absorpciometrijo ter določeni telesna teža, višina in indeks telesne mase. Med njimi je 107 preiskovank dovolilo tudi odvzem krvi zaradi določitve koncentracije serumskega estradiola. Z astereološko metodo smo v vsakem brisu na osnovi 200 izmerjenih ploščatih celic izračunali njihovo povprečno površino, povprečno površino citoplazme in jeder ter razmerje med jedrom in citoplazmo. Te vrednosti smo primerjali s T-vrednostmi, izmerjenimi na denzitometriji. Brise smo razdelili v atrofične in zrele ter jih prav tako primerjali s T-vrednostmi na kolku in ledveni hrbtenici. Rezultati: Povprečna površina celic in citoplazme je bila statistično značilno manjša pri preiskovankah z nižjimi T-vrednostmi tako na kolku kot na ledveni hrbtenici (p>0,05). Razmerje med jedrom in citoplazmo je bilo statistično značilno višje pri preiskovankah z nižjimi T-vrednostmi na kolku in ledveni hrbtenici (p<0,001). (Izvleček skrajšan pri 2000 znakih)
Descriptors		VAGINAL SMEARS CYTODIAGNOSIS BONE DENSITY ESTRADIOL BODY WEIGHT BODY HEIGHT BODY MASS INDEX OSTEOPOROSIS FEMUR NECK CERVIX UTERI