Author/Editor     De Martino, Sylvie Josiane; Sordet, Christelle; Piemont, Yves; Ružić-Sabljić, Eva; Thaddee Vetter, Marie; Monteil, Henri; Sibilia, Jean; Jaulhac, Benoît
Title     Enhanced culture of Borrelia garinii and Borrelia afzelii strains on a solid BSK-based medium in anaerobic conditions
Type     članek
Source     Res Microbiol
Vol. and No.     Letnik 157, št. 8
Publication year     2006
Volume     str. 726-9
Language     eng
Abstract     The growth of 29 human strains from the three main pathogenic species of Borrelia burgdorferi sensu lato on a solid BSK-based medium was compared in two culture atmospheres: 3% CO(2) air and anaerobiosis. All strains grew under anaerobic conditions, whereas only 13 strains were able to grow in aerobiosis with 3% CO(2) (P<0.001). In the latter condition, 75% of the B. burgdorferi sensu stricto strains grew versus 33% of the B. garinii and B. afzelii strains. These data suggest that, especially for B. garinii and B. afzelii species, anaerobic conditions enhance growth yield and speed of low-passage Borrelia strains.
Descriptors     BORRELIA
ANAEROBIOSIS
BACTERIOLOGICAL TECHNIQUES
CULTURE MEDIA