| Author/Editor | Gazić, Barbara | |
| Title | Napovedni pomen citometrične meritve DNA v vzorcu aspiracijske biopsije raka dojke | |
| Type | monografija | |
| Place | Ljubljana | |
| Publisher | Univerza v Ljubljani, Medicinska fakulteta | |
| Publication year | 2006 | |
| Volume | str. 107 | |
| Language | slo | |
| Abstract | In our retrospective study, the prognostic significance of flow cytometric DNA measurements was determined on fine needle aspiration biopsy (FNAB) samples of breast carcinoma. The prognostic significance of DNA ploidy and SPF was analysed in 770 breast cancer patients, the prognostic significance of aneuploid fraction in 480 aneuploid tumors and the prognostic significance of total nuclear protein content in 280 diploid tumors of breast cancer patients. The median follow up was 90 months (range 1-142, mean 87). During the time of follow up 366 patients (47.5%) had the recurrence of the disease and 283 patients (36.8%) died of disease. The cumulative 10-year overall cancer specific survival (OS) for all patients included In the study was 53.3% and the disease free survival (DFS) 44.5%. We morphometrically analysed the proportion of non-tumour cells in 139 FNAB smears of breast carcinoma and its influence on flow cytometric SPF estimation. The mean proportion of non-tumour cells in smears was 10.2%. Only in 10% of all samples, the proportion of nontumour cells exceed 20%, while in 70.5% of samples it was less than 10%. The difference between the proportion of non tumour cells in diploid and aneuploid tumours was not statistically significant. Lymhocytes and granulocytes represented the main type of non tumour cells. We corrected SPF values of diploid tumours for the dilution effect using indvidually established percentage of non-tumour cells (individual correction) and the mean proporion of non-tumour cells (factor correction). In a univariate analysis neither individual nor factor correction of SPF significantly changed its prognostic value for OS. (Abstract truncated at 2000 characters) | |
| Descriptors | BREAST NEOPLASMS NUCLEAR PROTEINS FLOW CYTOMETRY ANEUPLOIDY LYMPHATIC METASTASIS BIOPSY, NEEDLE FOLLOW-UP STUDIES SURVIVAL ANALYSIS NEOPLASM STAGING PROGNOSIS RETROSPECTIVE STUDIES |