| Author/Editor | Potokar, Maja; Kreft, Marko; Li, Lizhen; Andersson, J Daniel; Pangršič, Tina; Chowdhury, Helena H; Pekny, Miloš; Zorec, Robert | |
| Title | Cytoskeleton and vesicle mobility in astrocytes | |
| Type | članek | |
| Source | Traffic | |
| Vol. and No. | Letnik 8 | |
| Publication year | 2007 | |
| Volume | str. 12-20 | |
| Language | eng | |
| Abstract | Exocytotic vesicles in astrocytes are increasingly viewed as essential in astrocyte-to-neuron communication in the brain. In neurons and excitable secretory cells, delivery of vesicles to the plasma membrane for exocytosis involves an interaction with the cytoskeleton, in particular microtubules and actin filaments. Whether cyioskeletal elements affect vesicle mobility in astrocytes is unknown. We labeled single vesicles with fluorescent atrial natriuretic peptide and monitored their mobility in rat astrocytes with depolymerized microtubules, actin, and intermediate filaments and in mouse asirocytes deficient in the intermediate filament proteins glial fibrillary acidic protein and vimentin. In asirocytes, as in neurons, microtu6ules participated in directional vesicle mobility, and actin filaments played an important role in this process. Depolymerization of intermediate filaments strongly affected vesicle trafficking and in their absence the fraction of vesicles with directional mobility was reduced. | |
| Descriptors | ASTROCYTES CYTOSKELETON ATRIAL NATRIURETIC FACTOR ACTINS VIMENTIN NOCODAZOLE MICROTUBULE-ASSOCIATED PROTEINS CALCIUM TRANSFECTION POLYMERASE CHAIN REACTION MICROSCOPY, CONFOCAL MICE |