| Author/Editor | Eržen, Janez | |
| Title | Jedrne značilke pri nedrobnoceličnem pljučnem raku | |
| Type | monografija | |
| Place | Ljubljana | |
| Publisher | Univerza v Ljubljani, Medicinska fakulteta | |
| Publication year | 2006 | |
| Volume | str. 101 | |
| Language | slo | |
| Abstract | Image cytometric analysis offers a specific insight into DNA ploidy and chromatin organisation in the cell nucleus. When used in conjunction with generally accepted histological, immunohistological, genetic and molecular investigations, image cytometry provides additional information about the nature of the malignant cell, its biological features and prognosis of the disease. The method has found surprisingly little application in lung cancer research to date. In a third of patients with lung cancer, the disease will progress differently than expected on the basis of known facts. Therefore, we wanted to test whether image cytometry can provide additional information on non-small cell lung cancer (NSCLC). The aim of the study was to establish: - the structure and arrangement of chromatin in NSCLC cell nuclei; - the applicability of image cytometry for the estimation of prognosis and survival; - differences in DNA-ploidy and nuclear texture features among different stages and histological types; - differences in DNA-ploidy and nuclear texture features between living and deceased patients in the early and late stages of the disease; - differences in nuclear texture features between diploid and aneuploid tumours and their subtypes. A retrospective study of DNA-ploidy by image cytometry and nuclear texture features analysis was performed on 265 archival specimens of radically resected non-small cell lung carcinomas. Cell suspensions were prepared using the modified Hedley method, stained by the Feulgen-thionin method, and analysed using an automated image cytometer (Cyto-SavantTM). We found DNA-ploidy to be an independent prognostic factor in NSCLC, like disease stage. Two- and five-year disease specific survival rates were significantly better in patients with diploid tumors, especially in advanced stages of the disease (p = 0,04). (Abstract truncated at 2000 characters) | |
| Descriptors | LUNG NEOPLASMS CARCINOMA, NON-SMALL-CELL LUNG NEOPLASM STAGING PLOIDIES CELL NUCLEUS IMAGE CYTOMETRY PROGNOSIS CHROMATIN RETROSPECTIVE STUDIES |