Author/Editor		Hrastar-Kotešić, V; Hren-Vencelj, H
Title		Chlamydia trachomatis in men with nonspecific urethritis and evaluation of four tests for laboratory diagnosis
Type		članek
Source		Acta Dermatovenerol Alp Panon Adriat
Vol. and No.		Letnik 1, št. 4
Publication year		1992
Volume		str. 125-31
Language		eng
Abstract		In a study 162 men with nongonococcal urethritis (NGU) Chlamydia trachomatis CCT) infections were investigated. CT inclusions were assayed in cycloheximide-treated McCoy cells, chlamydial elementary boiiies lEB) by direct immunoiluorescent antibody method lDIF), by DNA hybridization assay and by indirect microimmunofluorescent detection (IIF) of IgA and IgG antibodies in serum. One hundred and eleven asymptomatic men were also tested and were considered as controls. CT was isolated from 43 patients with NGU (26,5 per cent ) and frorn 6 men (5,4 per cent ) ofthe control group l p less th. 0,05 ). The results obtained by DIF and DNA hybridization assay were compared with the isolations on McCoy cells, which was considered as the reference method. Sensitivity of the DIF turned out to be 87,5 per cent and it-s specificity 97,5 per cent , while the sensitivity of DNA hybridization assay was 26,5 per cent and it-s specificity 100 per cent . The positive predictive value lPPV) and negative predictive value for DIF were 92,1 and 96 per cent , the same values for DNA hybridization assay were 100 per cent and 77 per cent . The sensitivity of the serological tests for IgA and IgG antibodies were low (40 per cent and 44 per cent ), the specificity was adequate l87,3 per cent and 95.8 per cent ). PPV was 52,6 per cent and 78,5 per cent and NPV 80,7 per cent and 95.8 per cent respectively. All patients were treated with tetracyclines or with macrolides, after treatment control tests were carried out.
Descriptors		URETHRITIS CHLAMYDIA TRACHOMATIS CHLAMYDIA INFECTIONS DIAGNOSIS, LABORATORY