Author/Editor		Čižek-Sajko, Mojca; Vlaisavljević, Veljko; Kovač, Vilma
Title		Zorenje človeških jajčnih celic v pogojih in vitro
Translated title		Maturation of human oocytes in vitro
Type		članek
Source		Zdrav Vestn
Vol. and No.		Letnik 76, št. 1
Publication year		2007
Volume		str. 19-26
Language		slo
Abstract		Immature oocyte retrieval followed by in vitro maturation is a promising infertility treatment option. In patients with morphologically normal ovaries and regular menstrual cycles and in patients with polycystic ovary syndrome (PCOS) we attempted to assess the success of oocyte in vitro maturation in in vitro fertilization (IVF) procedures. Retrospectively we analyzed 87 1VF procedures with in vitro maturation of oocytes carried out in 73 infertile couples treated at the Maribor Teaching Hospital. We compared the success following three different hormone priming protocols: regular cycling patients with normal ovaries and without hormone priming (Group A, n=27); patients with PCOS and hormone priming with follitropin (follicle stimulating hormone, FSH) (Group 13, n=22); patients with PCOS and hormone priming with human chorionic gonadotrophin (hCG) (Group C, n=38). Success of the procedure was evaluated on the basis of the ability of oocytes to mature, fertilize and develop into embryos, and on the basis of the quality of embryos and their ability to implant in the uterus. In regular cycling patients with normal ovaries (n=27) we obtained a significantly lower number of immature oocytes (3.2 +- 2.5) compared with patients with PCOS and FSH priming (11, 7 +- 7.2) or those with PCOS and hCG priming (10.4 +- 7.2). The oocyte maturation rate, the fertilization rate and the embryo cleavage rate were as follows. in Group A 57.7% 63.2% and 91.7% in Group B 57.6%, 66,2% and 90.0%, and in Group C 58.0%, 66,2% and 91.0% (the differences between groups were not statistically significant). Six pregnancies were recorded only in patients with PCOS. The pregnancy rate per embryo transfer was 1/20 (5,0%) in patients with FSH priming and 5/33 (15,2%) in patients with hCG priming. (Abstract truncated at 2000 characters)
Summary		Pridobivanje nezrelih jajčnih celic in nato zorenje v pogojih in vitro je obetajoča metoda zdravljenja neplodnosti. Pri preiskovankah z morfološko normalnimi jajčniki in rednim menstruacijskim ciklusom ter pri preiskovankah s sindromom policističnih jajčnikov (PCOS) smo v postopkih zunajtelesne oploditve ugotavljali uspešnost zorenja jajčnih celic v pogojih in vitro. Pri 73 neplodnih parih smo retrospektivno analizirali 87 postopkov zunajtelesne oploditve z zorenjem jajčnih celic v pogojih in vitro. Primerjali smo uspešnost treh različnih protokolov hormonske priprave preiskovank: preiskovanke z normalnim menstruacijskim ciklusom in brez hormonskega spodbujanja (skupina A, n = 27); preiskovanke s PCOS in hormonsko pripravo s folitropinom (folikle spodbujajočim hormonom, FSH) (skupina B, n=22); preiskovanke s PCOS in hormonsko pripravo s humanim horionskim gonadotropinom (hCG) (skupina C, n=38). Uspešnost postopka smo ovrednotili s sposobnostjo jajčnih celic za zorenje, za oploditev in za razvoj v zarodke ter s kakovos jo zarodkov in njihovo sposobnostjo za ugnezditev v maternico. Pri preiskovankah z normalnim menstruacijskim ciklusom (n=27) smo dobili bistveno manj nezrelih jajčnih celic (3,2 +- 2,5) kot pri preiskovankah s PCOS in spodbujanih s FSH (11,7 +- 7,2) oziroma preiskovankah s PCOS in spodbujanih s hCG (10,4 +- 7,2). Stopnja dozorevanja jajčnih celic, stopnja oploditve in stopnja delitve zigot v zarodke je bila pri skupini A 57,7 %, 63,2 % in 91,7 %, pri skupini B 57,6%, 66,2% in 90,0%, ter pri skupini C58,0% 66,2% in 91,0% (razlike med skupinami so bile statistično neznačilne). Šest zanositev smo zabeležili le pri preiskovankah s PCOS. (Izvleček skrajšan na 2000 znakov)
Descriptors		POLYCYSTIC OVARY SYNDROME OOCYTES FERTILIZATION IN VITRO OVULATION INDUCTION FSH GONADOTROPINS, CHORIONIC EMBRYO TRANSFER PREGNANCY