Author/Editor		Ling, K-S; Zhu, H-Y; Petrovič, N; Gonsalves, D
Title		Serological detection of grapevine leafroll virus 2 using an antiserum developed against the recombinant coat protein
Type		članek
Source		J Phytopathol
Vol. and No.		Letnik 155
Publication year		2007
Volume		str. 65-9
Language		eng
Abstract		Grapevine leafroll associated viYUS 2 (GLRaV 2) is one of the important components in the leafroll disease complex. The coat protein gene of GLRaV 2 was cloned into a protein expression vector pMAL-c2x and the recombinant protein, consisting of the maltose binding protein (MBP) and GLRaV 2 coat protein (CP), was expressed in Escherichia coli. The recombinant MBP-CP was used to raise a h:gh quality antiserum. When used in Western blot analysis, the anti-MBP-CP antiserum produced specific reaction to the recombinant protein as well as to the viral coat protein of GLRaV 2. In Immunosorbent electron microscopy study, the anti-MBP-CP antibodies strongly decorated the GLRaV 2 virions. Using the newly developed antiserum, an indirect plate-trapped antigen enzyme-linked immunosorbent assay method was developed and successfully implemented for virus detection. A fteld survey was conducted to evaluate the virus infection status by GLRaV 2 and GLRaV 3 using antibodies developed against their respective recombinant coat proteins.
Descriptors		FRUIT CLOSTEROVIRUS MICROSCOPY, ELECTRON RECOMBINANT PROTEINS BLOTTING, WESTERN ENZYME-LINKED IMMUNOSORBENT ASSAY