| Author/Editor | Dolinšek, Jernej | |
| Title | Vpliv brezglutenske diete in genetskih dejavnikov na apoptotsko aktivnost sluznice ozkega črevesa pri otrocih s celiakijo | |
| Translated title | The effect of a gluten-free diet and genetic factors on apoptotic activity of the small intestine in children with celiac disease | |
| Type | monografija | |
| Place | Ljubljana | |
| Publisher | Univerza v Ljubljani, Medicinska fakulteta | |
| Publication year | 2006 | |
| Volume | str. 73 | |
| Language | slo | |
| Abstract | Background: Celiac disease is an immunologically mediated disease of the small intestine induced by gluten ingestion in genetically predisposed individuals. It can affect people of any age and cause many complications, of which malignant diseases are the most serious. The diagnosis of the disease is based on the assessment of a specific antibody pattern and histological changes of the intestinal mucosa, better known as vinous atrophy. The Marsh classification, used to determine the degree of mucosal damage, is based on the intraepithelial lymphocyte (IEL) count, crypt hyperplasia and vinous atrophy. The precise mechanisms of mucosal damage have not been definitively explained, but it is believed to be caused by a complex aberrant immune response to gluten in genetically predisposed individuals. Aim: The aim of our study was to determine whether enterocyte apoptosis is increased in children with active celiac disease compared to patients on a gluten-free diet. In addition, we sought to determine which predisposing genes are found in our celiac patients, and the correlation between enterocyte apoptosis and specific histological changes of the intestinal mucosa, especially the count of IEL carrying gamma/delta T-cell receptor (gamma/delta IEL) and specific serological changes, in particular antigliadin antibodies (AGA), endomysium antibodies (EMA), and tissue transglutaminase antibodies (t-TG). Our hypothesis was that enterocyte apoptosis is higher in genetically predisposed children with active celiac disease compared with patients on a gluten-free diet and controls. Methods: 15 children with active celiac disease, aged 5.84.1 years, 15 celiac patients on a gluten-free diet, aged 8.94.3 years, and 15 controls, aged 7.95.1 years, were included in the study. Serological markers (AGA, EMA, t-TG) were determined in all patients, as was the presence of HLA-DQ2 or HLA-DQ8. (Abstract truncated at 2000 characters) | |
| Descriptors | CELIAC DISEASE GLUTEN INTESTINE, SMALL APOPTOSIS T-LYMPHOCYTES ANTIBODIES HLA-DQ ANTIGENS IMMUNOHISTOCHEMISTRY CHILD |