Author/Editor | Bavec, Aljoša; Jiang, Yang; Langel, Ulo; Zorko, Matjaž | |
Title | Role of cysteine 341 and arginine 348 of GLP-1 receptor in G-protein coupling | |
Type | članek | |
Source | Mol Biol Rep | |
Vol. and No. | Letnik 34, št. 1 | |
Publication year | 2007 | |
Volume | str. 53-60 | |
Language | eng | |
Abstract | We have demonstrated the ability of peptides derived from the third intracellular loop of GLP-1 receptor to differently modulate activity of four different types of G-proteins overexpressed in sf9 cells. In this respect, the involvement of Cys(341) in inhibition of G(s) and Cys(341) in activation of G(s) and in inhibition of G(i1,) G(o), and G(11), respectively, indicates their potential role in discrimination between different types of G-proteins. Moreover, these two amino acids from the third intracellular loop might represent an important novel targets for covalent modification by downstream regulators in signaling through GLP-1 receptor. | |
Descriptors | GTP-BINDING PROTEINS RECEPTORS, GLUCAGON CYSTEINE ARGININE RECOMBINANT PROTEINS CELLS, CULTURED IMMUNOBLOTTING |