| Author/Editor | Retelj, Matjaž J; Harlander, Tatjana | |
| Title | Chromogenic media for urine cultures can be cost-effective | |
| Translated title | Kromogena gojišča za bakteriološko preiskavo urina so lahko stroškovno smotrna | |
| Type | članek | |
| Source | Zdrav Vestn | |
| Vol. and No. | Letnik 76, št. 3 | |
| Publication year | 2007 | |
| Volume | str. 145-9 | |
| Language | slo | |
| Abstract | Chromogenic media for diagnostic urinary bacteriology have several advantages over traditional media, such as cysteine-lactose-electrolyte deficient (CLED) medium. Chromogenic media allow for easier recognition of mixed growth, save time, reduce workload and provide higher detection rates. However, the cost of chromogenic media is significantly higher compared to CLED and performance of chromogenic media varies depending on the manufacturer. In the present study, performance, turn-around time and cost of Uriselect4 chromogenic medium was compared to CLFD. For performance analysis, 35Z midstream urine (MSU) samples from September 2005 to December 2005 were directly plated in parallel on Uriselect4 and CLED agar using the calibrated loop technique. Isolates on Uriselect4 were presumptively identified according to the product insert. Forcost-effectiveness analysis, we included1,972 consecutive MSU samples from May 2005 to July 2006. We compared the cost of required materials as well as technologists' or specialists' time for each medium examined. No significant differences were found between the isolation rates of urinary pathogens on the studied media. The procedure using chromogenic media for uropathogens is slightly cheaper than the procedure using CLED, considering the proportion of bacteriuria positive samples (50.5 %) and the distribution of taxes among isolates (namely Escherichia coli with 59.6%) observed in our laboratory. At the current isolation proportion in MSU samples processed in our laboratory, the average time to reporting results could be decreased by 0.3 days. Use of chromogenic media for urine investigations offers multiple advantages without increasing costs compared to procedures using CLED. (Abstract truncated at 2000 characters) | |
| Summary | Kromogena gojišča za bakteriološko preiskavo urina imajo številne prednosti pred tradicionalnimi gojišči, kot je gojišče s cisteinom in laktozo ter brez elektrolitov (CLED). Kromogena gojišča omogočajo lažje odkrivanje mešane rasti povzročiteljev okužb urinskih poti ter skrajšajo čas preiskave, zmanjšajo delovno intenzivnost ter imajo večjo stopnjo detekcije mikroorganizmov. Največja ovira pri uvedbi teh gojišč v rutinske postopke je njihova cena, saj je od 2- do 13-krat višja od cene gojišča CLED. Poleg tega se kromogena gojišča različnih izdelovalcev razlikujejo v zmogljivosti. Stroškovna smotrnost zamenjave tradi cionalnih gojišč s kromogenimi gojišči še ni bila podrobno raziskana. v tej raziskavi smo primerjali zmogljivost, trajanje in stroške postopka s kromogenim gojiščem Uriselect4 in postopka z gojiščem CLED. Za analizo zmogljivosti gojišča smo 351 zaporednih vzorcev srednjega curka urina od septembra 2005 do decembra 2005 neposredno cepili na gojišči Uriselect4 in CLED s tehniko kalibrirane zanke. lzolate na gojišču Uriselect4 smo identificirali skladno z navodilom za uporabo. V analizo stroškovne smotrnosti smo vključili 1972 zaporednih vzorcev srednjega curka urina od maja 2005 do julija 2006 Primerjali smo stroške potrebnega materiala ter potrebne delovne ure laboratorijskih tehnikov in mikrobiologov. Med gojiščema nismo odkrili statistično pomembnih razlik v stopnji izolacije povzročiteljev okužb urinskih poti. Postopek z uporabo kromogenega gojišča je nekoliko cenejši od postopka z uporabo gojišča CLED, če upoštevamo delež za bakteriurijo pozitivnih vzorcev srednjega curka urina (50,5%) in porazdelitev bakterijskih skupin med izolati (zlasti bakterije Escherichia coli z59,6% vseh izolatov) v našem laboratoriju. Pri tem deležu za bakteriurijo pozitivnih vzorcev srednjega curka urina se povprečno trajanje preiskave urina z uporabo kromogenega gojišča skrajša za 0,3 dneva. (Izvleček skrajšan na 2000 znakov) | |
| Descriptors | URINARY TRACT INFECTIONS CULTURE MEDIA BACTERIAL INFECTIONS URINALYSIS CHROMOGENIC COMPOUNDS COSTS AND COST ANALYSIS |