| Author/Editor | Sketelj, J; Črne-Finderle, N; Ribarič, S; Brzin, M | |
| Title | Interactions between intrinsic regulation and neural modulation of acetycholinesterase in fast and slow skeletal muscles | |
| Type | članek | |
| Source | Cell Mol Neurobiol | |
| Vol. and No. | Letnik 11, št. 1 | |
| Publication year | 1991 | |
| Volume | str. 35-54 | |
| Language | eng | |
| Abstract | 1. Initiation of subsynaptic sarcolemmal specialization and expression of different molecular forms of AChE were studied in fast extensor digitorum longus (EDL) and slow soleus (SOL) muscle of the rat under different experimental conditions in order to understand better the interplay of neural influences with intrinsic regulatory mechanisms of muscle cells. 2. Former junctional sarcolemma still accurnulated AChE and continued to differentiate morphologically for at least 3 weeks after early postnatal denervation of EDL and SOL muscles. In noninnervated regenerating muscles, postsynapticlike sarcolemmal specializations with AChE appeared (a) in the former junctional region, possibly induced by a substance in the former junctional basal lamina, and (b) in circumscribed areas along the whole length of myotubes. Therefore, the muscle cells seem to be able to produce a postsynaptic organization guiding substance, located in the basal lamina. The nerve may enhance the production or accumulation of this substance at the site of the future motor end plate. 3. Significant differences in the patterns of AChE molecular forms in EDL and SOL muscles arise between day 4 and day 10 after birth. The developmental process of downregulation of the asyrnmetric AChE forms, eliminating them extrajunctionally in the EDL, is less efficient in the SOL. The presence of these AChE forms in the extrajunctional regions of the SOL correlates with the ability to accumulate AChE in myotendinous junctions. The typical distribution of the asymmetric AChE forms in the EDL and SOL is maintained for at least 3 weeks after muscle denervation. 4. Different patterns of AChE molecular forms were observed in noninnervated EDL and SOL muscles regenerating in situ. In innervated regenerates, patterns of AChE molecular forms typical for mature muscles were instituted during the first week after reinnervation. 5.(trunc.) | |
| Descriptors | ACETYLCHOLINESTERASE MUSCLES SCIATIC NERVE SUPPORT, NON-U.S. GOV'T DENERVATION ENZYME INDUCTION MOTOR ENDPLATE MUSCLES NERVE REGENERATION RATS RATS, INBRED STRAINS REGENERATION SARCOLEMMA |