Author/Editor		Trošt, M
Title		Uporaba tokovne črpalke pri metodi vpete napetosti
Translated title		Current pump used by voltage-clamp method
Type		članek
Source		Med Razgl
Vol. and No.		Letnik 31, št. 4
Publication year		1992
Volume		str. 507-21
Language		slo
Abstract		By using the voltage-clamp method we can record the end-plate current which reflects changes in the conductance of the subsynaptic membrane during neuromusculartransmission. The classic (plain) voltage-clamp method shows some inherent deficiencies, which in some experimental conditions interfere with, or even prevent recording of the end-plate current. Therefore, the method was improved by attaching a "current pump" to the end of the feed-back loop. The aim of this work was to test the new mehtod of the "current pump assisted" voltage clamp in the curarized sartorius m. of the frog. Qualitatively the results are the same as those obtained by the "plain" voltage-clamp. However, the "current pump-assisted voltage-clamp is more stable and allows the use of a low-resistance current microelectrode. The set-up can be easily adapted for the classical method using two microelectrodes, as well as for microiontophoresis.
Descriptors		MOTOR ENDPLATE MEMBRANE POTENTIALS ACTION POTENTIALS MICROELECTRODES RANA TEMPORARIA ELECTRIC IMPEDANCE ELECTROPHYSIOLOGY