Author/Editor		Rakuša, Matej
Title		Biološka variabilnost in ponovljivost metod za merjenje delovanja trombocitov
Type		monografija
Place		Ljubljana
Publisher		Medicinska fakulteta
Publication year		2006
Volume		str. 40
Language		slo
Abstract		Background: Cardiovascular diseases are caused by disturbances in haemostasis. Haemostasis is a complex process, in which platelets play an important part. Only a few methods are available for assessing platelet function and are not standardized. Platelet aggregation is most commonly used. It is sensitive, but labour-intensive, time-consuming and expensive. Recent method of measuring closure time with platelet function analyser is relatively simple. Aim: To determine biological variability and precision of platelet aggregation and closure time, and sensitivity of both methods to platelet function disturbances caused by aspirin. Hypothesis: Precision of both methods for measuring platelet function is in expected range (coefficient of variability < 20%) and is comparable to other laboratory methods. Both methods are in agreement in establishing aspirin resistance. Methods: We included 20 healthy volunteers. Blood was drawn on two consecutive days without aspirin ingestion and on two consecutive days after 100 mg aspirin ingestion. In blood we measured hematocrit and number of platelets, platelet aggregation and speed of platelet aggregation with collagen, ADP and arachidonic acid (AK) and closure time with collagen/epinephrine (CEPI) and collagen/ADP (CADP) cartridges. Results: Precision for samples prior aspirin ingestion was 6% for platelet aggregation with ADP, 8% for aggregation with collagen, 5% for aggregation with AK, 16% for CEPI and 11 for CADP. (Abstract truncated at 2000 characters)
Descriptors		PLATELET FUNCTION TESTS PLATELET AGGREGATION ASPIRIN HEMATOCRIT PLATELET COUNT ARACHIDONIC ACID ADENOSINE DIPHOSPHATE COLLAGEN EPINEPHRINE REPRODUCIBILITY OF RESULTS