Author/Editor     Tomšič, Nejc
Title     Vpliv kardiolipina in holesterola na lastnosti fosfolipidnih mehurčkov in njihove interakcije z okolno raztopino
Type     monografija
Place     Ljubljana
Publisher     Medicinska fakulteta
Publication year     2006
Volume     str. 42
Language     slo
Abstract     Background. Negatively charged phospholipid membranes, microparticles and microvesicles represent catalytic surfaces for blood coagulation factors. Apart from blood coagulation factors, antiphospholipid antibodies (aPL) present in antiphospholipid syndrome (APS) play an important role in this process and increase the risk of blood cloth formation. Higher concentrations of microvesicles present in patients with APS further increases this risk. Giant phospholipid vesicles (GPVs) with controlled membrane composition present a simple in vitro system on which complex interactions between phospholipid membranes, microvesicles, microparticles and serum proteins can be studied. Upon the addition of reagents, changes in shape and dynamics of GPVs can be observed under the phase contrast optical microscope. Aim. Our aim was to study the impact of cardiolipin and cholesterol on the complex interactions between the membranes of GPVs, beta2-glikoprotein I ((beta2-GPI) and antibodies present in APS. For the observation of these interactions we wanted to set up a suitable system of GPVs containing cholesterol and high proportions of cardiolipin. Hypotheses. [beta2-GPI and anfiphospholipid antibodies (aPL) act as mediators in the interactions between GPVs with respect to membrane composition. Specific: (beta2-GPI and aPL mediate interactions between GPVs containing cardiolipin as a negatively charged phospholipid. (beta2-GPI also mediates interactions between GPVs without cardiolipn. Anti(beta2-GPI antibodies interact with GPVs only in the presence of (beta2-GPI. Monoclonal anti(beta2-GPI antibodies affect the interaction between (beta2-GPI and GPVs. (Abstract truncated at 2000 characters)
Descriptors     ANTIPHOSPHOLIPID SYNDROME
ANTIBODIES, ANTIPHOSPHOLIPID
ANTIBODIES, ANTICARDIOLIPIN
PHOSPHATIDYLCHOLINES
GLYCOPROTEINS
CARDIOLIPINS
CHOLESTEROL
GLYCOPROTEINS
MICROSCOPY, PHASE-CONTRAST