Author/Editor		Kotnik, V; Fleischmann, WR Jr
Title		A simple and rapid method to determine hematopoietic growth factor activity
Type		članek
Source		J Immunol Methods
Vol. and No.		Letnik 129
Publication year		1990
Volume		str. 23-30
Language		eng
Abstract		A rapid and simple colorimetric microassay method for the determination of hematopoietic growth factor activities was established. The assay was used to detect CSF-1, GM-CSF, and IL-3 activities. The assay was based on the metabolism of the tetrazolium dye 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide to formazan by metabolically active cells. Results obtained with the colorimetric microassay are comparable with those obtained with the soft agarose assay. Advantages of the colorimetric microassay include the conservation of reagents, the shorter incubation time for the experiment, the shorter assay time, and the ability to evaluate large numbers of samples.
Descriptors		COLORIMETRY GROWTH SUBSTANCES BONE MARROW CELLS, CULTURED COLONY STIMULATING FACTORS INTERLEUKIN 3 MICE, INBREAD C57BL RECOMBINANT PROTEINS REPRODUCIBILITY OF RESULTS SEPHAROSE TETRAZOLIUM SALTS THIAZOLES