Author/Editor		Breskvar, K; Črešnar, B; Plaper, A; Hudnik-Plevnik, T
Title		Localization of the gene encoding steroid hydroxylase cytochrome P-450 from Rhizopus nigricans inside a Hind III fragment of genomic DNA
Type		članek
Source		Biochem Biophys Res Commun
Vol. and No.		Letnik 178, št. 3
Publication year		1991
Volume		str. 1078-83
Language		eng
Abstract		The gene encoding steroid inducible cytochrome P450 of Rhizopus nigricans ATCC 6227b has been found inside a HindIII fragment of the genomic DNA by hybridization with a partial length cDNA probe. The latter was isolated by immunoscreening a cDNA library prepared in the lambda gt11 expression system and identified on the basis of inducibility and sequence analysis. The nucleotide sequence of the c DNA probe revealed a coding sequence for the heme binding segment characteristic of the P450 gene famlly.
Descriptors		CYTOCHROME P-450 DNA, FUNGAL GENES, STRUCTURAL, FUNGAL RHIZOPUS STEROID HYDROXYLASES AMINO ACID SEQUENCE BASE SEQUENCE DEOXYRIBONUCLEASE HIND III GENE LIBRARY MOLECULAR SEQUENCE DATA RESTRICTION MAPPING RHIZOPUS