Author/Editor     Milisav, I; Šuput, D
Title     Procaspase-9 is attached to the mitochondrial outer membrane in the early stages of apoptosis
Type     članek
Source     Cell Mol Biol Lett
Vol. and No.     Letnik 12, št. 4
Publication year     2007
Volume     str. 509-22
Language     eng
Abstract     Procaspase-9 is the zymogen form of one of the apoptosis initiators, caspase-9. Its cellular location may differ depending on the cell type; it is found throughout the cytosol, although some of it may be associated with the mitochondria. Procaspase-9 relocates from the cytosol to the mitochondria shortly after the triggering of apoptosis in rat hepatocytes. We investigated whether the mitochondrial protein import machineries import procaspase-9. The combined results of protein import analyses, mitochondrial fractionation and protease treatments of intact and swollen mitochondria imply that procaspase-9 attaches to the outer surface of the mitochondrial outer membrane.
Descriptors     APOPTOSIS
MITOCHONDRIA
PROTEIN BINDING
LIVER
CYSTEINE PROTEINASES
RATS
BIOLOGICAL TRANSPORT, ACTIVE