Author/Editor | Milisav, I; Šuput, D | |
Title | Procaspase-9 is attached to the mitochondrial outer membrane in the early stages of apoptosis | |
Type | članek | |
Source | Cell Mol Biol Lett | |
Vol. and No. | Letnik 12, št. 4 | |
Publication year | 2007 | |
Volume | str. 509-22 | |
Language | eng | |
Abstract | Procaspase-9 is the zymogen form of one of the apoptosis initiators, caspase-9. Its cellular location may differ depending on the cell type; it is found throughout the cytosol, although some of it may be associated with the mitochondria. Procaspase-9 relocates from the cytosol to the mitochondria shortly after the triggering of apoptosis in rat hepatocytes. We investigated whether the mitochondrial protein import machineries import procaspase-9. The combined results of protein import analyses, mitochondrial fractionation and protease treatments of intact and swollen mitochondria imply that procaspase-9 attaches to the outer surface of the mitochondrial outer membrane. | |
Descriptors | APOPTOSIS MITOCHONDRIA PROTEIN BINDING LIVER CYSTEINE PROTEINASES RATS BIOLOGICAL TRANSPORT, ACTIVE |