Author/Editor		Bobanović, F; Simčič, S; Kotnik, V; Vodovnik, L
Title		Pulsed electric current enhances the phorbol ester induced oxidative burst in human neutrophils
Type		članek
Source		Febs Lett
Vol. and No.		Letnik 311, št. 2
Publication year		1992
Volume		str. 95-8
Language		eng
Abstract		Oxidative burst (OB) response in human neutrophils, measured with chemilumincsccnce (CL). has been used to determine whether pulsed electric current (PEC) might induce a functional response in these electrically nonexcitabk cells, and also whether it might modify cellular response to tumor-promoting phorbol ester (PMA). Five minutes of PEC treatment caused no significant changes in neutrophil CL kvels in HBSS (1.2 m M Ca2+ concentration) as well as in HBSS-EGTA, where the extracellular Ca2+ concentration was reduced to less than 30 nM. The CL level of PMA-activated neutrophils in HBSS was 52 per cent higher than in HBSS-EGTA. In HBSS the CL kvel, after the combined PMA and PEC treatment, was 53 per cent higher than in PMA-alone-treated neutrophils. Activation of the OB in HBSS-EGTA with PMA and PEC was 13 per cent higher than in solely PMA treated neutrophils. The results suggest that in neutrophil OB response, the PEC effeet is closely related with cellular calcium mobilization, since depletion of extracellular Ca2+ decreased the PEC effect.
Descriptors		NEUTROPHILS ELECTRIC STIMULATION RESPIRATORY BURST CARCINOGENS PHORBOLS CALCIUM