Author/Editor     Coer, A; Legan, M; Štiblar-Martinčič, D; Čemažar, M; Serša, G
Title     Comparison of two hypoxic markers: pimonidazole and glucose transporter 1 (Glut-1)
Type     članek
Source     In: Kramar P, Zupanič A, Jarm T, editors. Medicon 2007. IFMBE proceedings from the 11th Mediterranean conference on medical and biological enginering and computing; 2007 Jun 26-30; Ljubljana. New York: Springer,
Publication year     2007
Volume     str. 465-8
Language     eng
Abstract     Tumour hypoxia occurs as a result of an inadequate supply of blood borne oxygen due to the disorganized and chaotic vascular network that develops in tumours. There is a recognized need for a method of measuring tumour hypoxia that is suitable for widespread clinical use. This problem may be partially overcome by the use of the bioreductive hypoxia marker pimonidazole; however, because the drug must be administered prospectively, studies on archival material are not possible. The presence of hypoxia in tumours results in the overexpression of certain genes, which are controlled via the hypoxia inducible factor-1 (HIF-1). The HIF-1-regulated protein glucose transporter 1 (Glut-1) has recently been introduced as an intrinsic marker of hypoxia. The aim of the current study was to compare the expression of Glut-1 with the binding of the bioreductive drug hypoxia marker pimonidazole and to elucidate the characteristics and pitfalls when they are used as a hypoxic marker. In the study, SA-1 solid subcutaneous tumours in A/J mice were treated by bleomycin given i.v. (1mg/kg), or the application of electric pulses (8 pulses, 1040 V, 100 micro s, 1Hz), or a combination of the two - electrochemo-therapy. Pimonidazole was injected 16 hours before tumour excision. The tumour were excised at different time points (0.5, 1 and 2 hours) after therapy. Immunohistochemistry for Glut-1 and pimonidazole adduct was carried out on two consecutive tumour sections and the percentages of positive staining areas were determined. Glut-1 staining was membranous and typically expressed peri-necrotically, whereas pimonidazole, although showing substantial co-localisation with Glut-1, was cytoplasmatic. Our results show that Glut-1 expression significantly correlates with the level of pimonidazole binding. (Abstract truncated at 2000 characters)
Descriptors     SARCOMA, EXPERIMENTAL
BLEOMYCIN
ELECTROPORATION
ANOXIA
MONOSACCHARIDE TRANSPORT PROTEINS
PIMOZIDE
IMMUNOHISTOCHEMISTRY
MICE, INBRED STRAINS
GLUCOSE