Author/Editor		Pretnar, G; Filipič, B; Golob, A; Škodič, A; Toth, S; Mecs, I; Suhar, A
Title		Electroinduction of interferon-like proteins
Type		članek
Source		Bioelectrochem Bioenerg
Vol. and No.		Letnik 320, št. 25
Publication year		1991
Volume		str. 183-93
Language		eng
Abstract		To investigate the phenomena of electroinduction, human leukocytes/lymphocytes were exposed to electric pulses with a frequency of 2 Hz, duration time of 0.5 micro s and voltage of 250 V, for a period of 30 min. After 18 h of incubation at 37 degrees C, the samples were collected and analyzed. The proteins found were very similar in their behaviour to interferons (antiviral activity against Herpes simplex virus type 1 in vitro, antiproliferative activity against transformed human cells, sensitivity to pH 2 and to a temperature of 56 degrees C), and very much alike in molar mass (45.000 D) to tumor necrosis factor (TNF). Their isoelectric point (4.3) showed a similarity to subtype IV of human alpha-interferon (IFN). In spite of the great similarity of the unknown proteins to cytokines, immuno blot tests with monoclonal antibodies against interferon alpha, acidolabile, gamma, TNF and interleukin 2 did not reveal their identity.
Descriptors		INTERFERONS ELECTRIC STIMULATION LEUKOCYTES CELLS, CULTURED