| Author/Editor | He, T; Nie, Y; Ge, Y; Xie, R; Yuan, T; Wang, Y; Galvani, Vesna; Rupreht, R; Čurin-Šerbec, Vladka | |
| Title | Expression of human-mouse chimeric antibody against TNF-alpha in CHO cells | |
| Type | članek | |
| Source | Chinese Journal of Biologicals | |
| Vol. and No. | Letnik 18, št. 5 | |
| Publication year | 2005 | |
| Volume | str. 368-70 | |
| Language | chi | |
| Abstract | Objective To construct human-mouse chimeric antibody against TNF-alpha and express in CHO cells. Methods Clone the gene encoding variable region of immunoglobulin from murine hybridoma cells by RT PCR and RACE methods. Construct human-mouse chirneric antibody by fusing the cloned gene with the gene encoding the constant region of human inununoglobulin . Transfect CHO cells with the fusion gene and detect the expressed product. Results The primary expression level of the constructed chimeric antibody in CHO cells was 0.71 microg/ml, and that after subclone was 0.95 microg/ml . Conclusion Anti-human TNF alpha chimeric antibody gene was successfully constructed and highly exp in CHO cells . | |
| Descriptors | CHIMERIC PROTEINS CHO CELLS TUMOR NECROSIS FACTOR CLONING, MOLECULAR HYBRIDOMAS POLYMERASE CHAIN REACTION IMMUNOGLOBULINS CHIMERIC PROTEINS |