Author/Editor		Sketelj, J; Črne, N; Brzin, M
Title		Molecular forms and localization of acetylcholinesterase and nonspecific cholinesterase in regenerating skeletal muscles
Type		članek
Source		Neurochem Res
Vol. and No.		Letnik 12, št. 2
Publication year		1987
Volume		str. 159-65
Language		eng
Abstract		Molecular forms and histochemical localization of acetylcholinesterase and nonspecičic cholinesterase were analysed in muscle regenerates obtained from rat EDL and soleus muscles after ischaemic-toxic degeneration and irreversible inhibition of preexistent enzymes. Regenerating myotubes and myofibres produce the 16S AChE form in the absence of innervation. The 10S AChE form prevails over 4S form with maturation into striated fibres. Although the patterns of AChE molecular forms in normal EDL and soleus muscles differ significantly no such differences were observed in noninnervated regenerates from both muscles. Two types of focal accumulation of AChE appear on the sarcolemma of regenerating muscles: first, in places of former motor endplates and, second, in extrajunctional regions. The 4S form of nonspecific cholinesterase is prevailing in regenerating myotubes whereas its asymmetric forms or focal accumulations could not be identified reliably. The satellite cells which survive after muscle degeneration probably originate from some type of late myoblasts and transmit the information concerning the ability to synthesize the asymmetric AChE forms and to focally accumulate AChE to regenerating muscle cells. Synaptic basal lamina from former motor endplutes may locally induce ACh E accumulatiuns in regenerating museles.
Descriptors		CHOLINESTERASES MUSCLES REGENERATION HISTOCYTOCHEMISTRY MOTOR ENDPLATE MUSCLES NEUROMUSCULAR JUNCTION RATS, INBRED STRAINS