Author/Editor		Lledo, PM; Vernier, P; Vincent, JD; Mason, WT; Zorec, R
Title		Inhibition of Rab3B expression attenuates Ca(2+)-dependent exocytosis in rat anterior pituitary cells
Type		članek
Source		Nature
Vol. and No.		Letnik 364, št. 6437
Publication year		1993
Volume		str. 540-4
Language		eng
Abstract		Low-molecular-weight GTP-binding proteins (small G proteins) of the Rab family have been proposed to act as central regulators of vesicular traffic, and proteins of the Rab3 subfamily (Rab3A, B, C and D) are thought to be associated with membrane vesicles or granules undergoing exocytotic fusion with the plasma membrane. Rab3A is highly expressed in brain, whereas Rab3B is the major form found in rat anterior pituitary gland. We report here that antisense oligonucleotides against Rab3B, introduced into pituitary cells using the whole-cell patch clamp technique, specifically and reversibly block expression of Rab3B. We find that calcium-dependent exocytosis is inhibited without affecting endocytosis. Antisense oligonucleotides directed against Rab3A have no effect. Our results indicate that Rab3B is likely to be a key intracellular signalling molecule which can control exocytosis downstream of other calcium-dependent processes in anterior pituitary cells.
Descriptors		CALCIUM EXOCYTOSIS G-PROTEINS NERVE TISSUE PROTEINS PITUITARY GLAND, ANTERIOR BASE SEQUENCE CELL MEMBRANE CELLS, CULTURED DNA ELECTROPHYSIOLOGY G-PROTEINS MOLECULAR SEQUENCE DATA NERVE TISSUE PROTEINS OLIGONUCLEOTIDE PROBES OLIGONUCLEOTIDES, ANTISENSE RATS