Author/Editor		Biček, A; Turel, I; Kanduser, M; Miklavčič, D
Title		Combined therapy of the antimetastatic compound NAMI-A and electroporation on B16F1 tumour cells in vitro
Type		članek
Source		Bioelectrochemistry
Vol. and No.		Letnik 71, št. 2
Publication year		2007
Volume		str. 113-7
Language		eng
Abstract		Ruthenium complex NAMI-A [ImH][trans-RuCl(4)(DMSO-S)Im] (Im = imidazole) is a potential chemotherapeutic drug in cancer treatment. Electroporation can be used to facilitate delivery of NAMI-A into cells. Suspension of B16F1 tumour cells from mouse melanoma in NAMI-A solution was exposed to a train of electric pulses. The effect of NAMI-A was determined by examining cell viability in clonogenic test. Our results show that electroporation increases the otherwise scarce in vitro effects of NAMI-A, i.e. reduces cell viability. At the conditions chosen for experiments 90% of cells survived in the presence of 1 muM NAMI-A, whereas in a combined treatment with 1 muM NAMI-A and electroporation only about 10% of cells survived.
Descriptors		TUMOR CELLS, CULTURED RUTHENIUM COMPOUNDS IMIDAZOLES NEOPLASM METASTASIS ELECTROPORATION DRUG DELIVERY SYSTEMS CELL SURVIVAL