| Author/Editor | Marc, MM; Korošec, P; Kern, I; Sok, M; Ihan, A; Košnik, M | |
| Title | Lung tissue and tumour-infiltrating T lymphocytes in patients with non-small cell lung carcinoma and chronic obstructive pulmonary disease (COPD): moderate/severe versus mild stage of COPD | |
| Type | članek | |
| Source | Scand J Immunol | |
| Vol. and No. | Letnik 66, št. 6 | |
| Publication year | 2007 | |
| Volume | str. 694-702 | |
| Language | eng | |
| Abstract | Cytotoxic CD8+ T cells have been suggested to be key players in the pathogenesis of chronic obstructive pulmonary disease (COPD). We wanted to investigate the phenotype of lung tissue T lymphocytes (LTL) and tumour-infiltrating T lymphocytes (TIL) in smokers with peripheral non-small cell lung carcinoma (NSCLC) with moderate/severe versus mild COPD. Lung tissue and tumour samples were obtained from patients with moderate/severe stage of COPD (n = 10) and from patients with mild stage of COPD (n = 7) at lung resection for a solitary peripheral NSCLC, processed and analysed by flow cytometry. The flow-cytometric results showed that lung tissue T cells, regardless of the severity of COPD, were mostly of the activated phenotype, expressed the CXCR3 chemokine receptor characteristic of type 1 T cells, and did neither significantly differ in the expression of activation markers (CD69, CD25 and HLA-DR), differentiation markers (CD27 and CD28) and chemokine receptors (CXCR3 and CCR4) between the selected groups, nor showed any significant correlation with lung function measured as forced expiratory volume in 1 s (FEV1) or TLCO. Compared with LTL, a significantly greater proportion of TIL expressed the activation markers CD69 and CD25, but a lower proportion showed a fully differentiated CD27- 28- phenotype. We conclude that lung LTL patterns are similar in NSCLC patients with moderate/severe or mild stages of COPD, and are not significantly related to lung function. LTL and TIL possess different phenotype characteristics. The majority of tumour tissue T cells are activated, but it seems that their process of differentiation is incomplete. | |
| Descriptors | LUNG NEOPLASMS CARCINOMA, NON-SMALL-CELL LUNG LUNG DISEASES, OBSTRUCTIVE LYMPHOCYTES, TUMOR-INFILTRATING T-LYMPHOCYTE SUBSETS KILLER CELLS FLOW CYTOMETRY IMMUNOPHENOTYPING |