Author/Editor		Jemec, Anita; Drobne, Damjana; Sepčić, Kristina; Tišler, Tatjana
Title		Uporaba biomarkerjev za določanje vplivov nanodelcev
Translated title		The use of biomarkers for assessing effects of nanoparticles
Type		članek
Source		Zdrav Vars
Vol. and No.		Letnik 46, št. 4
Publication year		2007
Volume		str. 144-50
Language		slo
Abstract		During the last decade, an enormous development of nanotechnologies has resulted in numerous improvements for our everyday lives. Nanomaterials (size at least 1-100 nm in one dimension) are new chemical forms of common chemical elements and exhibit unique physico-chemical and toxicological properties thatmay differ greatly from those of larger particles of the same materials. Until now, not many studies have focused on the effects of nanomaterials on humans and environment, so it is important to investigate their effects at several levels of organization. This paper presents our test system using terrestrial isopod Porcellio scaber as a model organism for the evaluation of the nanomaterials' effects on organisms. Animals were exposed to 15 nm titanium dioxide (TiO2) nanoparticles, and afterwards biochemical biomarkers, e.g. the activities of glutathione S-transferase and catalase, and physiological end-points (feeding rate, faeces production, food assimilation efficiency and animal mass change) were evaluated. An insignificant effect of TiO2 on feeding and significant effects on the activities of both enyzmes were noticed. The test system presented in this work proved to be quick, reliable and very informative. Further nano(eco)toxicological studies are recommended to enable the safe use of new nanomaterials.
Summary		V zadnjem desetletju smo priča izrednemu napredku nanotehnologij, ki so nam innam bodo v prihodnosti prinesle celo vrsto pridobitev za vsakdanje življenje. Poleg tega pa se moramo zavedati dejstva, da se bodo v našem okoljuv velikih količinah pojavljali novi nanomateriali (velikost vsaj 1-100 nm v eni dimenziji), ki imajo zaradi majhnosti drugačne fizikalno-kemijske in toksikološke lastnosti kot njihove večje oblike. O vplivih teh materialov na ljudi in okolje vemo zelo malo, zato je potrebno raziskati njihove morebitne vplive na različnih ravneh biološke organizacije. V prispevku smo predstavili testni sistem z mokrico Porcellio scaber za proučevanje vplivov nanomaterialovna organizme. Pri živalih, izpostavljenih 15 nm nanodelcem titanovega dioksida (TiO2), smo opazovali biokemijske biomarkerje, t.j. aktivnosti encimov glutation S-transferaze in katalaze, ter fiziološke biomarkerje (stopnja prehranjevanja, iztrebljanje, asimilacijska učinkovitost,sprememba mase živali). Opazili smo manjše vplive TiO2 na stopnjoprehranjevanja mokric in signifikantne spremembe aktivnosti obeh encimov. Menimo, da je predstavljeni pristop testiranja hiter in ponovljiv ternudi veliko informacij o mehanizmih delovanja nekega nanomateriala. Priporočamo nadaljnje nano(eko)toksikološke študije, ki nam bodo omogočile uporabo nanomaterialov s čim manjšimi posledicami za človeka in naše okolje.