| Author/Editor | | Injac, Rade; Perše, Martina; Bošković, Marija; Djordjević Milić, Vukosava; Djordjević, Aleksandar; Hvala, Anastazija; Cerar, Anton; Štrukelj, Borut |
| Abstract | | The therapeutic utility of the anthracycline antibiotic doxorubicin is limiteddue to its cardiotoxicity. Our aim was to investigate the efficacy of fullerenol C60(OH)24 in preventing single, high-dose doxorubicin-induced cardiotoxicity in rats with malignant neoplasm. Experiment was performed on adult female Sprague Dawley rats with chemically induced mammary carcinomas. The animals were sacrificed two days after the application of doxorubicin andžor fullerenol, and the serum activities of CK, LDH and Ž-HBDH, as well as the levels of MDA, GSH, GSSG, GSH-Px, SOD, CAT, GR, and TAS in the heart, weredetermined. The results obtained from the enzymatic activity in the serum show that the administration of a single dose of 8 mgžkg in all treated groupsinduces statistically significant damage. There are significant changes in the enzymes of LDH and CK (p < 0.05), after an i.p. administration of doxorubicinžfullerenol and fullerenol. Comparing all groups with untreated control group, point to the conclusion that in the case of a lower Ž-HBDHžLDH ratio, results in more serious the liver parenchymal damage. The results revealed that doxorubicin induced oxidative damage and that the fullerenol antioxidative influence caused significant changes in MDA, GSH, GSSG, GSH-Px, SOD, CAT, GR, and TAS level in the heart (p < 0.05). Therefore, it is suggested that fullerenol might be a potential cardioprotector in doxorubicin-treated individuals.
|