| Author/Editor | Režen, Tadeja; Juvan, Peter; Fon-Tacer, Klementina; Kuzman, Drago; Roth, Adrian; Pompon, Denis; Aggerbeck, L.P.; Meyer, Urs; Rozman, Damjana | |
| Title | The sterolgene v0 cDNA microarray: a systemic approach to studies of cholesterol homeostasis and drug metabolism | |
| Type | članek | |
| Source | BMC Genomics | |
| Vol. and No. | Letnik 9, št. 1 | |
| Publication year | 2008 | |
| Volume | str. 1-16 | |
| Language | eng | |
| Abstract | Background: Cholesterol homeostasis and xenobiotic metabolism are complex biological processes, which are difficult to study with traditional methods. Deciphering complex regulation and response of these two processes to different factors is crucial also for understanding of disease development. Systems biology tools such as microarrays can crucially contribute to this knowledge and can also discover novel interactions between the two processes. RESULTSČ We have developed a low density Sterolgene v0 cDNA microarray dedicated to studies of cholesterol homeostasis and drug metabolism in the mouse. To illustrate its advantages and evaluate its performance, we have analyzed mouse liver samples from studies focused on regulation of cholesterolhomeostasis and drug metabolism by diet, drugs and inflammation. Weobserved down-regulation of cholesterol biosynthesis during fasting and high-cholesterol diet and subsequent up-regulation by inflammation. Drug metabolism was down-regulated by fasting and inflammation, but up-regulated byphenobarbital treatment and high-cholesterol diet. Additionally, the performance of the Sterolgene v0 was also compared to the two commercial high density microarray platforms: the Agilent cDNA (G4104A) and the Affymetrix MOE430A GeneChip. We have hybridized the same RNA samples to the commercial platforms and show that the Sterolgene microarray performance is comparable interms of detection of changes in cholesterol homeostasis and drug metabolism. Conclusion: Using the Sterolgene v0 microarray we were able to detect important changes in cholesterol homeostasis and drug metabolism causedby diet, drugs and inflammation. Fundamentally, the Sterolgene series ofcDNA microarrays represents an original and dedicated tool, enabling focusedand cost effective studies of cholesterol homeostasis and drug metabolism. This tool also has the potential to be upgraded and developed as ascreening or diagnostic tool. | |
| Descriptors | CHOLESTEROL FASTING PHENOBARBITAL INFLAMMATION OLIGONUCLEOTIDE PROBES MICE STEROLS |