| Author/Editor | Kandolf-Sekulovic, Lidija; Kataranovski, Milena; Pavlovic, Milos D | |
| Title | Immunomodulatory effects of low-intensity near-infrared laser irradiation on contact hypersensitivity reaction | |
| Type | članek | |
| Source | Photodermatol Photoimmunol Photomed | |
| Vol. and No. | Letnik 19, št. 4 | |
| Publication year | 2003 | |
| Volume | str. 203-12 | |
| Language | eng | |
| Abstract | Background/purpose: Contact hypersensitivity (CHS) reaction is a useful model for studying the skin immune system and inflammatory reactions in the skin. In this study, an experimental model of CHS reaction was employed to assess immunomodulatory effects of near-infrared (near-IR) low-intensity laser (LIL) irradiation, which is used as adjuvant therapy in dermatology, physical medicine, rheumatology, etc., because of its declared anti-inflammatory, biostimulative and analgesic effects. Methods: The effects of near-IR LIL irradiation (lambda=904 nm, irradiance 60 mW/cm2, fluence 3.6 J/cm2) on CHS reaction to 1-chloro-2,4-dinitrochlorobenzene (DNCB) in Albino Oxford rats were examined by irradiating experimental groups of animals before the induction phase of CHS reaction, while nonirradiated animals and animals that received vehicle instead of hapten served as controls. Ear-swelling assay, histopathological examination of H&E preparations of ear skin, computer-assisted image analysis of dermal infiltrate, ear skin organ culture with the determination of cutaneous production of tumour necrosis factor-alpha (by ELISA assay) and nitric oxide (by Griess' assay) were used for measuring the effects of LIL in the elicitation phase of CHS reaction. Cellularity, dendritic cell content, flow cytometry and proliferation assays (spontaneous and in the presence of IL-2 and concanavalin A) of the draining lymph node cells (DLNC) were performed for the assessment of LIL irradiation effects in the induction phase. Results: In the irradiated group of animals, ear swelling was significantly diminished compared to control animals (101+/-11.5% vs. 58+/-11.6%, P<0.01). (Abstract truncate at 2000 characters) | |
| Descriptors | INFRARED RAYS ANIMALS DERMATITIS, ALLERGIC CONTACT DINITROCHLOROBENZENE EAR, EXTERNAL ENZYME-LINKED IMMUNOSORBENT ASSAY FLOW CYTOMETRY LYMPH NODES PHOTOTHERAPY RATS RATS, INBRED STRAINS STATISTICS, NONPARAMETRIC |