| Author/Editor | Kandolf-Sekulović, L; Cikota, B; Stojadinović, O; Bašanović, J; Skiljević, D; Medenica, Lj; Pavlović, M; Magić, Z | |
| Title | TCRgamma gene rearrangement analysis in skin samples and peripheral blood of mycosis fungoides patients | |
| Type | članek | |
| Source | Acta Dermatovenerol Alp Panonica Adriat | |
| Vol. and No. | Letnik 16, št. 4 | |
| Publication year | 2007 | |
| Volume | str. 149-55 | |
| Language | eng | |
| Abstract | Background: Diagnosing mycosis fungoides (MF) can be challenging in the early stage of the disease because histopathological features may simulate a variety of benign inflammatory skin diseases. Assessment of T-cell clonality was found to be useful in diagnosis and follow-up of patients. Objective: In this study, PCR-based TCRgamma gene rearrangement analysis was performed in skin and peripheral blood samples of patients with MF treated at the two largest referral centers in Serbia, and the results obtained were correlated with clinical and follow-up data. Methods: Skin and peripheral blood samples were obtained with informed consent from 37 patients treated at the Department of Dermatology of the Military Medical Academy and the Medical Center of Serbia from 2001 to 2006. The median time of follow-up was 4 years. Multiplex PCR was used for TCRgamma gene rearrangement analysis in skin and peripheral blood samples. Clonality results were correlated with the clinical data and disease course data. Results: Monoclonality was detected in skin samples of 30/37 patients (81%), in 2/5 patients with large-plaque parapsoriasis (LPP), in 28/32 (88%) patients with histologically proven MF, and in 1/16 (6%) patients with benign inflammatory dermatoses. A monoclonal pattern in both skin and peripheral blood was detected in 7/16 (44%) patients in the late stage of the disease, and in 1/7 (14%) patients in the early stage of the disease. A dominant clone was found in both skin and peripheral blood in 1/4 patients in remission, 2/5 with a stable disease, and 4/9 (44%) with disease progression. Conclusion: TCR-gamma gene rearrangement analysis can be regarded as a useful adjunct to diagnosis of epidermotropic lymphoproliferative disorders. (Abstract truncated at 2000 characters) | |
| Descriptors | GENE REARRANGEMENT, GAMMA-CHAIN T-CELL ANTIGEN RECEPTOR ADULT AGED AGED, 80 AND OVER DISEASE PROGRESSION MYCOSIS FUNGOIDES PARAPSORIASIS SKIN T-LYMPHOCYTES |