Author/Editor		Pan, Liangwen; Zhang, Shuya; Yang, Litao; Broll, Herman; Tian, Fenghua; Zhang, Dabing; Cankar, K; Vojvoda, Jana; Žel, Jana
Title		Interlaboratory trial validation of an event-specific quantitative polymerase chain reaction-based detection method for genetically modified RT73 rapeseed
Type		članek
Source		J AOAC Int
Vol. and No.		Letnik 90, št. 6
Publication year		2007
Volume		str. 1639-46
Language		eng
Abstract		The qualitative event-specific polymerase chain reaction detection method of genetically modified (GM) RT73 rapeseed was developed based on the cloned 3' end flanking sequence of RT73 rapeseed integration. The specificity of the method for GM RT73 rapeseed was validated using several different GM rapeseed lines, GM maize lines, GM soybean line, non-GM rapeseed, and other non-GM crops. In this study, the developed method was validated through an interlaboratory study by 12 laboratories from 6 countries. The sensitivity of this method was evaluated using several mixed rapeseed meals with different GM RT73 rapeseed contents from 5.0 to 0.01% prepared by our laboratory. The evaluated results showed that all of the rapeseed endogenous reference high mobility group protein gene (HMG I/Y), figwort mosaic virus 35S (FMV 35S) promoter, and RT73 event-specific fragment could be detected from rapeseed samples at 0.1% (w/w) with a confidence level of more than 95%. All results from the 12 laboratories indicated that the developed method could be considered fit for the detection and identification of GM RT73 rapeseed.
Descriptors		CROPS, AGRICULTURAL POLYMERASE CHAIN REACTION OLIGONUCLEOTIDE PROBES QUALITY CONTROL INTERNATIONAL COOPERATION PLANTS, TRANSGENIC