Author/Editor		Strle, Klemen; Zhou, Jian-Hua; Broussard, Suzanne R; Venters, Homer D; Johnson, Rodney W; Freund, Ggregory G; Dantzer, Robert; Kelley, Keith W
Title		IL-10 promotes survival of microglia without activating Akt
Type		članek
Source		J Neuroimmunol
Vol. and No.		Letnik 122, št. 1-2
Publication year		2002
Volume		str. 9-19
Language		eng
Abstract		IL-10 is an anti-inflammatory cytokine that has recently been shown to promote survival of neurons and glia. Here we establish that IL-10 induces phosphorylation of Stat3 on Tyr(705) and serves as a survival factor for N13 microglial cells. Recombinant IL-10 (10 ng/ml) decreases growth factor withdrawal-induced apoptosis by 50%, as assessed by TUNEL. In contrast to IL-10, IGF-I increases enzymatic activity of PI 3-kinase and causes phosphorylation on serine(473) of Akt but does not prevent microglial apoptosis. These data establish that IL-10 activates Stat3 and inhibits the mitochondrial pathway of cell death without activating the Akt cell survival pathway.
Descriptors		PROTEIN-SERINE-THREONINE KINASES ANIMALS APOPTOSIS CELL DIVISION CELL SURVIVAL CELLS, CULTURED DNA-BINDING PROTEINS ENZYME ACTIVATION INSULIN-LIKE GROWTH FACTOR I INTERLEUKIN-10 MICE MICROGLIA PHOSPHORYLATION PROTO-ONCOGENE PROTEINS RECEPTORS, INTERLEUKIN SERINE TETRAZOLIUM SALTS THIAZOLES TRANS-ACTIVATORS TYROSINE