Author/Editor		Strle, Klemen; McCusker, Robert H; Tran, Lynn; King, Alexandra; Johnson, Rodney W; Freund, Gregory G; Dantzer, Robert; Kelley, Keith W
Title		Novel activity of an anti-inflammatory cytokine: IL-10 prevents TNFalpha-induced resistance to IGF-I in myoblasts
Type		članek
Source		J Neuroimmunol
Vol. and No.		Letnik 188, št. 1-2
Publication year		2007
Volume		str. 48-55
Language		eng
Abstract		IL-10 is an anti-inflammatory cytokine that suppresses synthesis of proinflammatory cytokines and their receptors. Here we tested the possibility that TNFalpha-induced hormone resistance in myoblasts might be overcome by IL-10. We found that IL-10 restores myogenesis by suppressing the ability of exogenous TNFalpha to inhibit IGF-I-induced myogenin. This protection occurs without decreasing global activity of TNF receptors since IL-10 does not impair TNFalpha-induced IL-6 synthesis or ERK1/2 phosphorylation. Instead, IL-10 acts to prevent TNFalpha-induced phosphorylation of JNK. These findings demonstrate that IL-10 serves a previously unrecognized protective role in muscle progenitors by overcoming TNFalpha-induced resistance to IGF-I.
Descriptors		ANALYSIS OF VARIANCE ANIMALS CELL LINE DOSE-RESPONSE RELATIONSHIP, DRUG DRUG INTERACTIONS ENZYME-LINKED IMMUNOSORBENT ASSAY GENE EXPRESSION REGULATION INSULIN-LIKE GROWTH FACTOR I INTERFERON-ALPHA INTERLEUKIN-10 INTERLEUKIN-6 MICE MYOGENIN