Author/Editor		Vukojevic, Katarina; Carev, Dominko; Sapunar, Damir; Petrovič, Danijel; Saraga-Babic, Mirna
Title		Developmental patterns of caspase-3, bax and bcl-2 proteins expression in the human spinal ganglia
Type		članek
Source		J Mol Histol
Vol. and No.		Letnik 39, št. 3
Publication year		2008
Volume		str. 339-49
Language		eng
Abstract		The distribution of the bcl-2, bax and caspase-3 proteins was investigated in the cells of developing human spinal ganglia. Paraffin sections of 10 human conceptuses between 5th and 9th gestational weeks were analysed morphologically, immunohistochemically and by TUNEL-method. Cells positive to caspase-3 had brown stained nuclei or nuclear fragmentations. At earliest stages, 6% of ganglion population were caspase-3 positive cells. Later on, a significant increase in number of caspase-3 positive cells appeared, particularly in the ventral part of ganglia (12%), and subsequently decreased to 6%. TUNEL-positive cells had the same distribution pattern as caspase-3 positive cells. Bax-positive cells followed the developmental pattern similar to caspase-3 cells, changing in range between 20% and 32%. There were 8% of bcl-2 positive cells at earliest stages. They increased significantly in dorsal part of the ganglion during the 7th week (28%), and than dropped to 15% by the end of the 8th week. These findings suggest a ventro-dorsal course of development in human spinal ganglia. Number of bcl-2, bax and caspase-3 positive cells changed in a temporally and spatially restricted manner, coincidently with ganglion differentiation. While apoptosis might control cell number, bcl-2 could act in suppression of apoptosis and enhancement of cell differentiation.
Descriptors		GANGLIA, SPINAL APOPTOSIS PROTO-ONCOGENE PROTEINS C-BCL-2 CYSTEINE PROTEINASES DNA FRAGMENTATION IMMUNOHISTOCHEMISTRY GESTATIONAL AGE EMBRYO